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    Documents | Using the Biotage® Horizon 3100 and In-Line Pacific® Sodium Sulfate Drying Cartridges for EPA Method 1664B

    Using the Biotage® Horizon 3100 and In-Line Pacific® Sodium Sulfate Drying Cartridges for EPA Method 1664B

    The purpose of this application note is to provide a streamlined workflow solution for measuring hexane extractable material (HEM) using the Biotage® Horizon 3100 for solid phase extraction and Pacific® Sodium Sulfate Drying Cartridges for in-line drying. Using the Speed-Vap®, the extracts will be concentrated with gentle heat and consistent air flow through precisely drilled holes.

    3100

    Introduction

    The treatment and removal of oil and grease from wastewater is imperative because it can negatively affect the biological and aquatic life that encounter it. Not only that, but since the oil and grease is not miscible with water, it leaves an unappealing layer on top of the water. Even before the oil and grease can reach the aquatic life, it will solidify on the inner walls of pipes, causing blockage over time.

    Conclusion

    This application note demonstrates that HEM can be effectively recovered within the guidelines of EPA Method 1664B with the use of Oil and Grease product solutions from Biotage. In addition to that, the blanks indicate virtually no contamination of HEM from consumables or instrumentation. Automated solid-phase extraction improves the precision of HEM recoveries by eliminating operator bias. With the option of the Pacific®  Drying Cartridge, installed in-line with extraction, it reduces operator interaction with the sample extracts, but also stays within compliance of EPA Method 1664B.

     

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