Documents | Using Focused Gradients to Improve Peptide Purity Using Reversed-Phase Flash Chromatography

Using Focused Gradients to Improve Peptide Purity Using Reversed-Phase Flash Chromatography

The use of reversed-phase high performance flash chromatography (HPFC) is gaining traction as a strategy to reduce the overall purification time for synthetic peptides. Herein we present data demonstrating that a focused gradient, with a slope reminiscent to that commonly used for HPLC-mediated peptide purification, can indeed be used in HPFC to improve the final purity of a desired peptide with minimal time invested in method development.

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Biotage is the Global Go-To Separations Company, supporting customers from drug discovery and development through to diagnostics and analytical testing with intelligent and sustainable workflow solutions. Our expertise and top-tier separation solutions play a key role in streamlining our customers’ workflows and improving their outcomes. Headquartered in Sweden, Biotage operates globally with 700 employees, serving over 80 countries. Our company is listed on NASDAQ Stockholm (BIOT).

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Using Focused Gradients to Improve Peptide Purity Using Reversed-Phase Flash Chromatography