Drinking water is one of the primary sources of human exposure to toxic chemicals. The U.S. EPA identifies and regulates a number of compounds in drinking water that could pose health risks, and outlines methods for properly quantifying them. Contaminants can be biological, physical, chemical or radiological, and can exist in a wide range of concentrations. Therefore, the list of EPA methods that are approved for use in testing drinking water is extensive and each method presents its own challenges, based on the specific compounds being quantified.
EPA Method 525.2 is used to quantitate organic compounds found in drinking and source waters. Method 525.2 uses a reversed phase separation mechanism to isolate a large variety of compounds from the sample matrix. The reversed phase separation is achieved using a C18 bonded silica stationary phase which is packed into an SPE cartridge or disk. The C18 stationary phase allows for the extraction of semi-volatile compounds from the sample matrix which are then analyzed by gas chromatography-mass spectrometry (GC-MS).1
Download the full application note describing the use of Atlantic® ReadyDisk HC-C18 SPE disks for the automated extraction of EPA 525.2 analytes from drinking water, using the Biotage® Horizon 5000 extraction system.