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    Documents | Extraction of Opiates from Whole Blood Using ISOLUTE® SLE+ Prior to GC/MS Analysis

    Extraction of Opiates from Whole Blood Using ISOLUTE® SLE+ Prior to GC/MS Analysis


    This application note describes the extraction of opiate compounds from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, barbiturates, benzodiazepines and cocaine. ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 50 ng/mL (analyte dependant).

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