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    Documents | Extraction of Fat-Soluble Vitamins from Human Serum Using ISOLUTE® SLE+ Prior to UHPLC/MS-MS Analysis

    Extraction of Fat-Soluble Vitamins from Human Serum Using ISOLUTE® SLE+ Prior to UHPLC/MS-MS Analysis


    This application note describes the extraction of a panel of fat soluble vitamins (including those representing Vitamins A, D, E & K) from human serum using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries approximately or above 90% with RSDs 10% or less for all analytes.

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