Extraction of a Comprehensive Steroid Panel from Human Urine Using ISOLUTE® SLE+ Prior to LC/MS-MS Analysis

This application note describes the extraction of a panel of 19 steroid hormones from human urine using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS-MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 90% with RSDs lower than 10% for all analytes. Linearity of greater than 0.999 is achieved for all analytes in the range 1-1000 pg/mL. Manual and automated (using Biotage Extrahera) extraction protocols are detailed.