Extraction of a Comprehensive Steroid Panel from Human Serum Using EVOLUTE® EXPRESS ABN Prior to LC/MS-MS Analysis

This application note describes the extraction of a panel of 19 steroid hormones from human serum using EVOLUTE® EXPRESS ABN solid phase extraction plates prior to LC/MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 10% for the majority of analytes. Linearity of >0.99 is achieved for all analytes in the range 5-5000 pg/mL. The application note includes details on automation of the extraction method using Biotage® Extrahera.