Determining Organic Compounds in Drinking Water Utilizing the Biotage® Horizon 5000 and TurboVap® II

This application note outlines a procedure for the extraction and concentration of organic compounds in drinking water utilizing the Biotage® Horizon 5000, TurboVap® II with End-Point Sensors, and Atlantic® C18 SPE Disks. To demonstrate compliance, the limits set in EPA Method 525.21 were used as a guideline and an initial demonstration of capability (IDOC) as well as method detection limit (MDL) study was performed.

When SPE is performed manually, unintentional errors can occur throughout the process. The same can be said when concentrating extracts, such as inconsistent final volumes or uneven nitrogen delivery into sample tubes. These factors can end up in skewed results and relative standard deviations, resulting in possible method criteria failures. The proposed solution outlined below will eliminate manual intervention throughout the extraction process while delivering a very reliable, efficient and consistent means of concentrating while demonstrating its ability to comply to the EPA Method 525.2 guidelines.