Automated Clean-up of Pesticides in Broccoli using ISOLUTE® cSPE for QuEChERS

This application note describes the extraction of a panel of 81 commonly analysed pesticides from broccoli using a two-step QuEChERS workflow. Samples are first homogenized and extracted with ISOLUTE® EN QuEChERS extraction salts using the Biotage® Lysera™ bead mill homogenizer. Following centrifugation, an aliquot of extract is cleaned up using ISOLUTE® cSPE for QuEChERS columns, processed using a Biotage® Extrahera™ automated sample preparation workstation. Extracts are evaporated using TurboVap™ Dual 96 and analysis is performed using LC-MS/MS.