Automated analysis of a Panel of Fat Soluble Vitamins A, D, E and K in Serum by Supported Liquid Extraction and LC-MS/MS Analysis

Simultaneous fat-soluble vitamin testing can be challenging for a variety of reasons: solubility limitations of the analytes, high levels of protein binding and wide variation of concentration ranges from one vitamin to another. This poster investigates extraction optimization strategies to provide a method for the simultaneous analysis of Vitamin A (Retinol and Beta Carotene), Vitamin D (25-OH D2 and D3), Vitamin E (Alpha Tocopherol) and Vitamin K (Phylloquinone K1, and Menaquinone K2). Samples could be processed manually or on the automated Extrahera™ platform. AACC 2021