Clinical and Bioanalytical Sample Preparation

Simultaneous extraction of fat soluble vitamins (A, D, E and K) from serum presents a number of challenges, from the diversity of molecular structures and properties, to the wide variation in relevant clinical concentrations. Biotage specialists developed a method based on the Supported Liquid Extraction technique that effectively addresses these challenges.

Vitamin B7 (Biotin) is a water-soluble vitamin necessary for cell growth. The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum while minimizing co-extractable material in the form of proteins, lipids and phospholipids. 

Extraction method of a comprehensive panel of steroid hormones from human serum using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS-MS analysis.

A robust, mixed-mode weak cation exchange SPE protocol for the extraction of catecholamine and metanephrine metabolites from urine prior to LC-MS/MS detection. Fully automatable  for high throughput assays, the method can prepare 96 samples in 52 minutes.

This white paper examines a number of aspects of sample preparation, and their impact on the success of subsequent LC-MS/MS analysis of broad urine panels. Originally published in 2017, the white paper has now been updated to include details of additional hydrolysis enzymes and optimum hydrolysis conditions.