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When it comes to the purification of polar, water-soluble compounds reversed-phase chromatography is the most commonly used approach. However, because of strong stationary phase – mobile phase repulsi...

In my role as senior technical specialist at Biotage I am often asked about compound detection options. For most flash chromatography methods, UV is the default detection tool since many compounds do ...

Applying green chemistry principals to flash purification is becoming increasingly important. In this post, I discuss ways to make flash column chromatography greener by reducing solvent use through o...

In previous posts I have touched upon various sample loading options and how they impact flash chromatographic performance, primarily in normal-phase flash purification. As the use of reversed-phase f...

This is an age-old question that has been around a long time, perhaps as long as me (and I have been around a while) – “Does silica dissolve in methanol?”

When it comes to the purification of polar organic compounds many chemists turn to normal-phase flash chromatography with dichloromethane and methanol as the mobile phase. This solvent system often ca...

Compound detection challenges are, for many chemists, a part of life. In a previous post I discussed how wavelength focusing can help your flash system detect and fractionate compounds with poor chrom...

Mass spectrometers today are typically available with either Electrospray Ionization (ESI) or APCI (Atmospheric Pressure Chemical Ionization) sources. That’s really nice but, how do you know which sou...

Have you ever experienced compound tailing or streaking on your TLC plate or flash chromatography results and wondered what in the world is going on here? Well, there can be multiple reasons for this ...

Compounds precipitating during flash chromatography is at best an inconvenience when working up your crude reaction mixture. Precipitation during purification typically happens in the column or in the...