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Gradient or isocratic elution, that is the question. Within flash chromatography these are the options afforded to separate reaction mixtures and natural product extracts and is the focus of this post...

Gradients, used in chromatographic methods, assist with chemical separation and elution. They begin with “weak” elution conditions and end with “strong” elution conditions.

A fairly common question I receive is "how much of my reaction mixture can I load on my flash chromatography column?". To this question there is not a cut and dry answer because of a number of variabl...

Chromatography is as much an art as it is a science. Between synthetic reaction products and natural products, the range of compounds requiring separation, purification, and isolation is broad and div...

In previous posts I offered some suggestions to improve the “greenness” of normal-phase flash purification. As the use of reversed-phase flash purification has increased the past few years I thought I...

Reversed-phase flash chromatography use continues to increase for a variety of reasons. Unlike silica normal-phase flash columns, which typically are used only once, reversed-phase flash columns can b...

For many labs on a tight budget, keeping expenses at a minimum is crucial. Many of these same labs are also required to become more sustainable, or greener, by reducing the amount of organic solvent t...

Have you ever been faced with the challenge of evaporating solvent from a reaction mixture where the target compound and perhaps some by-products are temperature sensitive and degrade or decompose wit...

Chemists using silica columns for normal-phase flash chromatography typically equilibrate their columns prior to loading their samples. Companies manufacturing automated flash purification systems oft...

With all forms of chromatography there are limitations relating to sample load – both mass and volume. These are independent variables which, for the best results, should be investigated separately. I...