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Using double coupling to improve your peptide synthesis
Small Molecules and Synthetic Therapeutics
Double coupling costs not only time, but also extra reagents. Is it really necessary though? Read more to find out!
Peptide purification with flash column chromatography - a beginner's experience
Small Molecules and Synthetic Therapeutics
Read about my first time trying out reversed phase flash chromatography for purifying my peptides. Spoiler alert: it works!
Can I improve my peptide purification by increasing the column length?
Small Molecules and Synthetic Therapeutics
Have you ever used a longer HPLC column to improve your separation? Read more to find out if this is a good strategy for flash ...
Using Mixed Stationary Phases to Improve Your Peptide Purification with Flash Chromatography
Small Molecules and Synthetic Therapeutics
Sometimes crude peptides are so dirty they require 2 purification passes. Read more to learn how connecting two different columns can ...
Cyclic Peptides Webinar Recap
Small Molecules and Synthetic Therapeutics
It's clear that cyclizing peptides can, not only increase proteolytic stability, but may also increase cell permeability. As a result, ...
How to improve peptide purification by altering the mobile phase pH
Small Molecules and Synthetic Therapeutics
Flash chromatography is often disregarded for peptide purification given its lower resolution. Read more to learn how alternative ...
Optimizing the removal of an ivDde protecting group
Small Molecules and Synthetic Therapeutics
Learn about an optimized protocol for automated synthesis and ivDde protecting group removal using the Initiator+ Alstra™ peptide ...
Using microwave heating to expedite your allyl ester or alloc deprotection
Small Molecules and Synthetic Therapeutics
Comparing conditions to expdite the selective removal of ally or alloc ester protecting groups using microwave heating.
Disulfide Rich Peptides - which order should the disulfide bonds be formed?
Small Molecules and Synthetic Therapeutics
When synthesizing peptides that contain multiple disulfide bonds you may consider the order in which those bonds are formed.
Room temperature allyl ester and alloc deprotections - what is the lifetime of palladium?
Small Molecules and Synthetic Therapeutics
Compare conditions for the selective removal of ally or alloc ester protecting groups at room temperature. Is it suitable for automation?
