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How to use a scouting column for your peptide purification
Small Molecules and Synthetic Therapeutics
Predicting peptide elution profile is critical for efficient peptide purifications. Learn how a scouting column, with matched stationary ...
Post synthesis workup: What steps are necessary and what aren't?
Small Molecules and Synthetic Therapeutics
Learn how to quickly and gently evaporate peptide cleavage cocktail, reducing your total peptide workflow time significantly.
How does methanol as a mobile phase solvent impact peptide purification by reversed-phase flash chromatography?
Small Molecules and Synthetic Therapeutics
Methanol is increasingly used for reversed phase purification in place of acetonitrile. Learn how methanol impacts your peptide ...
How long should I let my cleavage reaction stir at room temperature?
Small Molecules and Synthetic Therapeutics
Cleaving peptides from resin is often a very fast reaction, however side chain protecting groups may require more time. What reaction time ...
Optimizing a mobile phase gradient for peptide purification using flash column chromatography
Small Molecules and Synthetic Therapeutics
Reversed phase flash chromatography is growing in popularity as a strategy to purify crude peptide samples. Read more to how gradient ...
How to purify peptides using a step gradient in flash column chromatography
Small Molecules and Synthetic Therapeutics
Step gradients are often used in normal phase chromatography. Read more to learn how they can be used to also improve the purity of your ...
Increasing your peptide purity using a focused gradient with flash chromatography
Small Molecules and Synthetic Therapeutics
Peptide purification with flash chromatography can be hampered by resolution losses. Read more to learn how a focused gradient can improve ...
How does my mass spectrometer carrier solvent impact mass-directed purification of peptides?
Small Molecules and Synthetic Therapeutics
Ionization efficiency is critical for effective mass directed purification of peptides. Read more to learn how the carrier solvent impacts ...
How do I choose my resin for peptide synthesis? {Part 2}
Small Molecules and Synthetic Therapeutics
Resin choice is critical to ensure a successful peptide synthesis. Learn how swelling capacity and general hydrophilicity can help improve ...
How do I choose my resin for peptide synthesis? {Part 1}
Small Molecules and Synthetic Therapeutics
Resin choice can play a large role in successful peptide synthesis. Learn more about how resin substitution levels can impact your ...
