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Which Stationary Phase Should I Chose For My Peptide Purification?
Small Molecules and Synthetic Therapeutics
Choosing a stationary phase seems relatively straightforward, almost automatic even. See how selectivity differences could impact your ...
How to choose an ion pairing agent to improve your peptide purification
Small Molecules and Synthetic Therapeutics
Sometimes closely eluting impurities are difficult to resolve by simply changing the gradient. Learn how ion pairing agents can help.
How to purify your peptide using mass directed flash chromatography
Small Molecules and Synthetic Therapeutics
Mass directed purification simplifies purification efforts. Read more how it can be used to reduce your purification time with flash ...
Peptides containing cysteine: the role of scavengers in cleavage cocktail
Small Molecules and Synthetic Therapeutics
In this blog, Beth highlights the role of of scavengers for peptides containing cysteine residues.
How much peptide is recovered from a reversed-phase C18 cartridge during flash purification?
Small Molecules and Synthetic Therapeutics
Everyone asks about recovery when considering new strategies for peptide purification. Read more to learn about recovery when using flash ...
How does mobile phase modifier concentration impact peptide purity with flash chromatography?
Small Molecules and Synthetic Therapeutics
Learn how acidic modifier concentration can impact your peptide purification with flash chromatography.
How to choose your acidic mobile phase modifier for peptide purification using Reversed-Phase flash chromatography
Small Molecules and Synthetic Therapeutics
Mobile phase modifier choice can impact selectivity of peptides for the stationary phase. Read more about using a buffer to modify your ...
How to use the isoelectric point to inform your peptide purification mobile phase pH
Small Molecules and Synthetic Therapeutics
The pH of a peptide isolectric point is a useful reference when evaluating how to handle these compounds.
How to purify hydrophilic peptides - Why won't my peptide stick to my column?
Small Molecules and Synthetic Therapeutics
Purifiying hydrophilic peptides present a unique challenge for reversed phase chromatography. Read more how injection solvent can impact ...
How to synthesize hydrophobic peptides - Choosing the Right Solvent
Small Molecules and Synthetic Therapeutics
The overall hydrophobicity of a peptide can severely limit the synthesis success. Read more to learn how choosing an alternative system ...
