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Extractable Petroleum Hydrocarbons: What are they & how are they monitored
Extractable Petroleum Hydrocarbons (EPH) may be invisible, but their impact on the environment is tangible. Through advanced monitoring techniques and a robust regulatory framework, we strive to strik...
What is Acrylamide?
In the world of science, there are always new topics and compounds that are discovered, but not all of them get much publicity. One of these is acrylamide. Now you’re probably saying to yourself, “wha...
Extracting both acidic and basic analytes in a single SLE procedure
Often the question arises asking how can I extract both acids and bases with the same Supported Liquid Extraction (SLE) procedure. Is this possible? And how?
Does media choice matter for peptide library purification using solid phase extraction?
Unbiased peptide screening technologies have evolved, improved and recently gained acceptance by the community as strategies for identifying new lead compounds against old targets with in novel chemic...
What is the difference between solid vs. liquid particles in ELSD signal?
Preparative HPLC and flash chromatography are two of the most utilized techniques for purifying molecules post synthesis. To monitor sample purity and elution times, detection techniques such as UV/UV...
How does the make-up solvent impact Evaporative Light-scattering detection?
Evaporative light-scattering detection (ELSD) is an effective tool for detecting compounds with minimal to no UV absorbance. ELS detectors convert the column effluent into an aerosol by adding heated ...
Purifying natural product extracts? Why you should use ELSD with UV detection
I like working with natural products, at least from a chromatography perspective. The sheer breadth of compounds extractable from a plant makes separations a challenge but overcoming that challenge wi...
Why you should add an ELSD to your flash chromatography system
ELSD, or evaporative light scattering detector, is an accessory item available for many commercially available flash chromatography systems. It is typically used to detect compounds that are UV transp...
Can Coupling Reagents Cause Premature Cleavage from Cl-Trityl Resin?
Cl-trityl resin can be tricky to use not only from an amino acid loading perspective, but one must also consider the highly labile linkage to the solid support1. This lability is advantageous in insta...