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Does dry load media choice impact reversed-phase flash purification results?
Small Molecules and Synthetic Therapeutics
This post compares the purification results of 5 dry load media vs. liquid loading.
What is my C18 flash column's loading capacity?
Small Molecules and Synthetic Therapeutics
This post describes methodology to determine reversed-phase column loading capacity.
Increasing sample load for peptide purification with a step gradient
Small Molecules and Synthetic Therapeutics
Has your chromatogram changed dramatically when you increase sample load? Read more to learn about factors to consider for peptide ...
How to decrease your time for peptide purification optimization
Small Molecules and Synthetic Therapeutics
Method development for peptide purification can be time consuming, especially when the guess-and-check method is your only option. Improve ...
Optimizing the removal of an STmp protecting group
Small Molecules and Synthetic Therapeutics
Read our blog to learn more about one protection strategy that can be used for these compounds.
Green solvents for solid phase peptide synthesis
Small Molecules and Synthetic Therapeutics
Green chemistry solutions are highly desirable. Read more to learn about greener solvent alternatives to DMF and NMP for peptide synthesis.
What mobile phase flow rate should I use for my peptide purification with flash chromatography?
Small Molecules and Synthetic Therapeutics
Flow rate affects both selectivity and resolution. Read more to help determine which flow rate works best for purifying peptides with flash ...
How many amino acid equivalents should I use for my room temperature synthesis?
Small Molecules and Synthetic Therapeutics
Amino acids can account for a significant fraction of the total cost for peptide synthesis. Read more to learn how many equivalents you can ...
Automating stapled peptide synthesis: overcoming DMF poisoning
Small Molecules and Synthetic Therapeutics
Fully automating synthesis and secondary chemistry is always desirable, unless it comes at a cost of yield or purity. Read more for ...
How does flow rate affect my peptide purification efficiency when using a small pore stationary phase
Small Molecules and Synthetic Therapeutics
Particle size and pore size can both impact resolution, but especially when using reversed phase flash chromatography. Read more to see ...
