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How does reagent concentration impact synthesis?
Small Molecules and Synthetic Therapeutics
This post discusses the impact that reagent concentration has on a synthetic product yield and purity.
How Much Is My Flash Chromatography System Really Going to Cost?
Small Molecules and Synthetic Therapeutics
How much does it cost? One of the first questions when considering an automated flash column chromatography system. Here we answer the ...
How to quantify your first amino acid loading onto Wang resins
Small Molecules and Synthetic Therapeutics
Occassionally, peptide chemists still have to couple the first amino acid onto Wang-type resins. Read more to learn how to quantify the ...
How does gradient slope impact flash chromatography loading capacity?
Small Molecules and Synthetic Therapeutics
This post shows how gradient slope optimization increases sample loading.
How to Purify your Target Compound with Minimal Impurities
Small Molecules and Synthetic Therapeutics
This post describes various flash chromatography options available for targeted compound purification.
What is the best way to purify a crude reaction mixture that auto-crystallizes?
Small Molecules and Synthetic Therapeutics
This post discusses the benefits of purifying the full precipitated reaction mix vs. filtration, phase separation, redissolution and ...
What are alternatives to DCM/MeOH for polar reaction mix purifications?
Small Molecules and Synthetic Therapeutics
This post describes alternatives to DCM/Methanol for polar compound purification.
Does dry load media choice impact reversed-phase flash purification results?
Small Molecules and Synthetic Therapeutics
This post compares the purification results of 5 dry load media vs. liquid loading.
What is my C18 flash column's loading capacity?
Small Molecules and Synthetic Therapeutics
This post describes methodology to determine reversed-phase column loading capacity.
Increasing sample load for peptide purification with a step gradient
Small Molecules and Synthetic Therapeutics
Has your chromatogram changed dramatically when you increase sample load? Read more to learn about factors to consider for peptide ...
