When a peptide is synthesized and cleaved from a resin, the next step is usually a quick preparation and multiple injections onto a preparative HPLC column for isolation from deletion sequences and other contaminants. A great step to take before preparative HPLC is a single injection onto a flash purification system. This quick clean-up is often sufficient to move to the next step in the synthesis. Flash purification prior to preparative HPLC also creates the opportunity to further purify the peptide of interest with fewer injections and a less challenging mixture, and extends the life of expensive preparative HPLC columns.

• Enables fast and efficient purification of
  peptides and larger molecules.
• Increased loading capacity compared to prep RP-HPLC which allows more peptide to be processed in a single injection.
• Reduction in solvent usage and therefore
  waste compared to RP-HPLC.
• Depending on the quality of the peptides synthesized, flash chromatography can be adopted in the workflow either as the sole method of purification or as a front end clean-up prior to RP-HPLC.
• Our flash chromatography solutions have been used to purify peptides, PNA, peptoids and peptidomimetics.