Speed up peptide purification using flash chromatography

Purification is a key bottleneck in peptide synthesis workflows. Here are the benefits of high-performance flash chromatography in peptide workflows:

Handle larger sample sizes with higher loading capacity than RP-HPLC
Reduce waste and costs with less solvent usage
Optimize your workflow with versatility for both primary purification and pre-RP-HPLC clean-up
Purify a wide range of peptides, including PNA, peptoids, and peptidomimetics

5 tips for successful peptide purification

Improve your peptide flash purification with practical tips for better results and faster workflows, plus expert guidance on optimizing gradients.

Peptide workflow at Red Glead Discovery

Find out how Red Glead Discovery improves efficiency with flash purification. Some peptides achieve high purity in a single run, while others require additional RP-HPLC purification. The greatest time savings are seen with longer peptides.

Application note: Accelerate peptide purification with flash chromatography

Discover how flash chromatography can serve as the primary purification method for crude synthetic peptides, helping you speed up your workflow. Read Part 1 and Part 2.

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Flash systems for peptide purification

The Biotage® Selekt automated flash purification systems are optimized for fast, efficient peptide purification with a range of lab-scale columns with a choice of reversed-phase media.

Flash columns for peptide purification

Biotage® Sfär flash columns enable high-performance reversed-phase purification of peptides, developed with small particles and larger pores for efficient bulk purification or enrichment before RP-HPLC.

Biotage insights: Small molecules & synthetic therapeutics

Does media choice matter for peptide library purification using solid phase extraction?

SPE is emerging as a strategy for peptide library clean up in parallel.

What is the difference between solid vs. liquid particles in ELSD signal?

ELSD is useful for finding molecules that have no chromophore during flash purification. Here we show how signal intensity can vary between compounds!

How does the make-up solvent impact Evaporative Light-scattering detection?

This post describes how ELSD make-up solvent choice can impact detected peak sensitivity.

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Product groups

Biotage® Selekt

A two column channel system with a colorful and intuitive user interface on a large touchscreen enables even the most complex tasks to become simplicity itself. High flowrate and pressure make separation faster and greener due to less solvent consumption.

Isolera™ Dalton 2000

The Isolera™ Dalton 2000 system consists of Biotage® Dalton 2000 Mass Detector, Isolera™ Dalton Nanolink and Isolera™ Spektra for efficient and automated purification. Isolera Dalton 2000 directs flash fractionation by molecular mass, and in real time.

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Biotage® Sfär Bio C4 D

High performance flash chromatography is a very efficient technique to clean-up synthetic peptides. Biotage® Sfär Bio flash columns, with a small particle size (20 μm) and large pore size (300 Å), provide increased resolution and effective separation of complex peptide mixtures. C4 is more suitable for more hydrophobic peptides.

Other steps in the workflow

Removing Peptide Cleavage Cocktail and evaporating DMSO is no longer a problem with Biotage® V-10 Touch.

Evaporation of Peptides

Biotage offer a range of manual, semi-automated and fully automated systems depending on your peptide synthesis needs. Whether you require a system that makes a single peptide or multiple peptides in parallel, we have an instrument to suit your requirements.

Peptide Synthesis