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AAV PhyTip columns for purification of Adeno Associate Viruses
Adeno-associated virus (AAV) is a non-enveloped virus that can be engineered to deliver DNA to a target. The genome of this virus consists of a single-stranded linear DNA molecule of approximately 4.7 kb. The Adeno-associated virus is used as a vector for the delivery of various types of cargo to the cytoplasm and nucleus of various cells including muscle, vascular endothelial and neuronal cells.
For the downstream workflow of virus production, affinity chromatography purification has been found to effectively remove impurities such as plasmid DNA and host cell RNA/DNA. That is then followed by an anion exchange polish to remove empty capsids.
The CaptureSelectTM AAVX affinity matrix is specifically designed to address the high selectivity and capacity requirements for large-scale downstream purification of a broad range of naturally occurring and synthetic adeno-associated virus (AAV) serotypes used for gene therapy applications. CaptureSelectTM AAVX resin has demonstrated binding reactivity towards a set of AAV serotypes that includes AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, and AAVrh10 as well as synthetic serotypes.
The CaptureSelectTM AAVX PhyTip® columns with our proprietary Dual Flow Chromatography are compatible with most 8, 12, and 96-channel automation liquid handling robots.
CaptureSelect® is a trademark of Thermo Fisher Scientific.
PhyNexus, now part of Biotage, developed the PhyTip® column in 2002 and have supported researchers with automated protein purification ever since. PhyTip columns purify samples by repeated back-and-forth cycles - a process called dual flow chromatography which allows complete binding interactions in each purification step.
Our AAV PhyTip columns are packed with a high capacity AAV affinity resin, which routinely generates purified samples of his-tagged protein with 60 - 80% sample recovery.
Our unique column design with a packed resin bed results in binding of the entire sample to a small resin bed volume. The purified sample can be eluted in small volumes, resulting in a highly concentrated sample.
PhyTip columns process samples by dual flow chromatography which is a gentle purification process for high levels of biologically active protein.
Effective purification by PhyTip columns enables a full purification protocol of 96 samples in as little as 15 minutes.
Our Protein A PhyTip columns comes with dual flow chromatography protocols that have be validated with real protein and can be used on any automated liquid handling system.
