This application note describes a simple ISOLUTE® PPT+ protocol for the extraction of methylmalonic acid (MMA) from serum, demonstrating high, reproducible analyte recoveries with low protein content in the extracts.
ISOLUTE PPT+ Protein Precipitation plates offer a fast and
extremely simple sample preparation approach. Serum proteins
are efficiently removed from the sample using an in-plate,
‘solvent first’, protein crash and filter procedure, which is
easily automated using the Biotage Extrahera system. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags: 96-well plate, Acidic, Application Notes, Biomarker, Clinical, English, Extrahera, MMA (Methylmalonic acid), Methylmalonic acid (MMA), Serum
This Application Note reviews the use of ISOLUTE PPT+ plates for the isolation of drugs from biological fluids, including the method, recovery data and the impact of the filtrate’s cleanliness on analyte quantitation.
Tags: Application Notes
•Extends the range of acidic, basic and neutral compounds that can be extracted using a single generic method, increasing method development productivity•Has an optimized pore structure which reduces the extraction of endogenous matrix components, and leads to cleaner extracts that give fewer matrix effects in LC-MS/MS analysis•Has optimized physical and chemical characteristics that improve reliability of SPE procedures•Has no secondary interactions, allowing the use of pure organic elution solvent to give high recoveries of very low drug concentrations
Tags: Manuals & User Guides
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
Tags: Biological fluids, English, HPLC, LC-MS/MS, Plasma, Posters
Protein precipitation is a routinely used sample preparation technique for removal of proteins from biological fluid samples prior to analysis. Protein precipitation in the 96-well format, using filtration for protein removal, is a high throughput alternative to the traditional centrifugation based technique.
Tags: English, Product Notes
Protein precipitation is a routinely used, high throughput sample preparation technique for removal of protein from biological fluid samples prior to analysis by LC-MS/MS.
Tags: Biological fluids, Plasma, Product Notes
Biotage® PRESSURE+ manifolds deliver positive pressure, parallel processing for 96 well plates, 1 mL 3 mL and 6 mL column formats. The systems utilize a consistent, uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns.
Biotage® VacMaster™-96 sample processing manifold has been designed with the needs of the bioanalytical scientist in mind. This manifold is ideal when processing 96-well SPE, supported liquid extraction or protein precipitation plates either manually or with an automated liquid handling system.
|Part No.||Description||Product type||Pack Size||Price|
|120-2040-P01||ISOLUTE® PPT+ Fixed Well Plate||Consumables||1||Log in for price|
|121-5202||Collection Plate, 1 mL Square||Consumables||50||Log in for price|
|121-5203||Collection Plate, 2 mL Square||Consumables||50||Log in for price|
|121-5204||Piercable Sealing Cap||Consumables||50||Log in for price|
|121-5213||Collection Plate, 2 mL Round||Consumables||50||Log in for price|