Remove More Phospholipids - Get Better Sensitivity
ISOLUTE® PLD+ plates and columns deliver cleaner extracts than other phospholipid removal products, meaning lower detection limits can be achieved. In the example below, signal-to-noise using ISOLUTE PLD+ was more than double when compared with competitor products (figure 1), leading to an immediate enhancement in sensitivity.
Figure 1. Relative peak intensity, 20 pg/mL amitriptyline in plasma (Blue = ISOLUTE® PLD+, Orange = Competitor O, Red= Competitor P).
Excellent Protein and Phospholipid Removal
More than 99 % of phospholipids are removed as the sample passes through the multifunctional sorbent bed, leaving clean, purified extracts for analysis (figure 2). Removing phospholipids also improves analytical column lifetime, preventing the build-up of phospholipids over multiple injections, and maintaining analyte sensitivity over extended analytical runs.
Figure 2. Phospholipids in human plasma treated by a) protein precipitation and b) ISOLUTE® PLD+. Lysophospholipids and phospholipids present in plasma treated by protein precipitation (red and green respectively) are shown in 1b on an expanded scale for direct comparison with residual levels of lysophospholipids and phospholipids present in ISOLUTE® PLD+ treated plasma (purple and blue respectively).
Effective Sample Clean-up in 3 Simple Steps
Figure 3. Typical ISOLUTE PLD+ procedure
Using a simple solvent crash/filtration based procedure, proteins and phospholipids are simultaneously removed from plasma and other blood based samples while high, reproducible analyte recoveries are maintained. The optimised frit arrangement acts as a depth filter, efficiently trapping precipitated proteins, without blocking or plugging.
How to Use ISOLUTE® PLD+ Protein and Phospholipid Depletion Plates
How to Use ISOLUTE® PLD+ Protein and Phospholipid Depletion Columns
Trouble Free Processing
ISOLUTE® PLD+ plates and 'tabless' columns have excellent flow characteristics, and do not plug or block during use. They can be processed easily using either positive pressure or vacuum based systems, and are compatible with most automated liquid handlers.
Up to 96 x 1 mL volume tabless format columns can be processed on Biotage® Pressure+ 96, Extrahera and VacMaster-96 systems using specially designed racks. This cost effective option is ideal for applications with variable sample numbers or for method development.
Find out more about ISOLUTE PLD+ products, along with handy tips for improving your results with our FAQs