|Chemical description||Amino/Octadecyl layer|
|Sorbent type||Layered column|
This application note describes the extraction of polyaromatic hydrocarbons (PAHs) from natural waters with a high concentration
of humic acids. Layered SPE columns are used to eliminate humic acids from the final extract.
Tags: Application Notes, Benzo(a)anthracene, Benzo(a)pyrene, Benzo(b)fluoranthene, Benzo(ghi)perylene, Benzo(k)Fluoranthene, Chrysene, Column, English, Environmental, Fluoranthene, Fluorene, Polyaromatic Hydrocarbons (PAH), Polyaromatic hydrocarbons, Water
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The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
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What concentration of PAH's do I get after strain by means of ISOLUTE 1,5 g PAH filter if i have passed through it 500 mL sample?
This is an open ended question as it depends on the initial concentration of the PAH compounds in the water sample and the final volume of your finished extract both of which are unknown. Let me outline with a couple of examples.
Water sample contains various PAHs at 5 ng/L per component
We extract 500 mL through the ISOLUTE PAH column
We elute the analytes from the column using a suitable solvent and then concentrate the resulting eluent
If we concentrated down the eluent to 1 mL final volume we have achieved a x 500 concentration factor and analytes are now present in the final solution at 2.5 ng/mL.
If we concentrated down the eluent to 0.1 mL final volume we have achieved a x 5000 concentration factor an analytes are now present in the final solution at 25 ng/mL.Browse more questions and answers >
What is the maximum capacity of an SPE column?
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.Browse more questions and answers >