|Chemical description||Amino/Octadecyl layer|
|Sorbent type||Layered column|
This application note describes the extraction of polyaromatic hydrocarbons (PAHs) from natural waters with a high concentration
of humic acids. Layered SPE columns are used to eliminate humic acids from the final extract.
Tags: Application Notes, Benzo(a)anthracene, Benzo(a)pyrene, Benzo(b)fluoranthene, Benzo(ghi)perylene, Benzo(k)Fluoranthene, Chrysene, Column, English, Environmental, Fluoranthene, Fluorene, Polyaromatic Hydrocarbons (PAH), Polyaromatic hydrocarbons, Water
In this catalog product information is displayed with easy-to-follow application details and supporting documentation.
Ordering information is found following each product listing.
The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Tags: Manuals & User Guides
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This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE® EPH column in conjunction with the Biotage&re...20 February 2017
This application note describes the extraction of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized the for ...05 January 2016
Now available, the Biotage 2016 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...11 August 2015
Dilute and shoot (D&S) methods are promoted for use by analytical instrument vendors in clinical, forensic toxicology and bioanalytical laboratories. More sensitive LC-MS/MS systems make this s...27 March 2015
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...08 January 2015
Now available, the Biotage 2015 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...29 July 2014
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
What concentration of PAH's do I get after strain by means of ISOLUTE 1,5 g PAH filter if i have passed through it 500 mL sample?
This is an open ended question as it depends on the initial concentration of the PAH compounds in the water sample and the final volume of your finished extract both of which are unknown. Let me outline with a couple of examples.
Water sample contains various PAHs at 5 ng/L per component
We extract 500 mL through the ISOLUTE PAH column
We elute the analytes from the column using a suitable solvent and then concentrate the resulting eluent
If we concentrated down the eluent to 1 mL final volume we have achieved a x 500 concentration factor and analytes are now present in the final solution at 2.5 ng/mL.
If we concentrated down the eluent to 0.1 mL final volume we have achieved a x 5000 concentration factor an analytes are now present in the final solution at 25 ng/mL.Browse more questions and answers >
What is the maximum capacity of an SPE column?
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.Browse more questions and answers >