|Chemical description||Carboxylic acid modified polystyrene-divinylbenzene incorporating non-ionisable hydroxyl groups|
|Sorbent type||Mixed-mode non-polar/weak cation exchange|
|Exchange capacity||0.4 mmol/g|
|Average particle size||30 µm, 50 µm|
|Pore diameter||40 Å|
This application note describes a mixed-mode weak cation exchange SPE protocol for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) and three metanephrine metabolites (metanephrine, normetanephrine and 3-methoxytyramine) from urine prior to LC-MS/MS detection.
Included in this application note are the detailed settings for implementing the method on the Biotage® Extrahera(TM) automation system.
Tags: 3-methoxytyramine, Application Notes, Catecholamines, Clinical, Dopamine, English, Epinephrine, Extrahera, LC-MS/MS, Metanephrine, Metanephrines, Norepinephrine, Normetanephrine, Urine
This application note describes a mixed-mode weak cation exchange solid phase extraction protocol using EVOLUTE® EXPRESS WCX 96-well plates for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) from human plasma prior to LC-MS/MS detection, which allows low level quantification of the analytes.
Tags: 96-well plate, Application Notes, Catecholamines, Clinical, Dopamine, English, Epinephrine, LC-MS/MS, Norepinephrine, Plasma
The method described in this application note achieves high recoveries of normetanephrine, metanephrine, and 3-methoxytyramine from hydrolysed urine using EVOLUTE® WCX solid phase extraction products.
Tags: 3-methoxytyramine, 96-well plate, Application Notes, Catecholamines, Clinical, English, Hydrolysed urine, LC-MS/MS, Metanephrine, Normetanephrine
This application note describes a polymer based-based weak exchange mixed-mode SPE protocol for the extraction of the aminoglycoside antibiotics gentamicin, streptomycin, spectinomycin and neomycin from chicken meat prior to LC-MS/MS analysis. Linearity and LOQ meet USDA-FSIS guidelines.
Tags: Aminoglycoside antibiotics, Antibiotics, Application Notes, Chicken meat, Column, English, Food, Gentamicin, LC-MS/MS, Neomycin, Spectinomycin, Streptomycin
This application note describes a method of extraction for metanephrine and normetanephrine from pooled
plasma using EVOLUTE® EXPRESS WCX 96-well SPE plates.
A new solid phase extraction
technology has been developed to reduce the time
associated with sample preparation methods by eliminating
traditional steps in the extraction workflow. Leveraging this
technology, a method protocol was developed using a load,
wash and elute approach. This method was optimized for
Tags: 96-well plate, Application Notes, Catecholamines, Clinical, English, LC-MS/MS, Metanephrine, Normetanephrine, Plasma
In this catalog product information is displayed with easy-to-follow application details and supporting documentation.
Ordering information is found following each product listing.
EVOLUTE® EXPRESS Solid Phase Extraction products
from Biotage are designed for all your sample
preparation challenges. Whether you are working in the field of bio-analysis during drug development, clinical or forensic
toxicology, or with the diversity of samples in food
safety and environmental applications, the tools and
methods used for sample preparation have a clear
impact on productivity. This user guide contains information about choosing the correct EVOLUTE EXPRESS product for your application and optimizing your methodology for best results.
Tags: 96-well plate, Clinical, Column, English, Environmental, Food, Forensic, Manuals & User Guides, On-line SPE, Pharma
Whether working in the field of bio-analysis in
drug development, clinical or forensic analysis, or
with the diversity of samples in food safety and
environmental applications, the tools and
methods used for sample preparation have a
clear impact on productivity during method
development, validation and routine analysis.
EVOLUTE SPE sorbents and extraction
methodologies have been developed to extract a
wide range of analytes while reducing or
eliminating matrix components that can cause ion
suppression in LC-MS analysis.
Tags: Clinical, Environmental, Food, Forensic, Manuals & User Guides, Pharma
This poster discusses the impact of optimization of various parts of the method development process to maximize assay performance while delivering a combined assay for the analysis of urinary catecholamines and metanephrines. The extraction method using EVOLUTE EXPRESS WCX 96-well plates is successfully automated using the Biotage® Extrahera™ Automated Sample Preparation Platform.
MSACL 2017, Palm Springs
Tags: 3-methoxytyramine, 96-well plate, Catecholamines, Clinical, Dopamine, English, Epinephrine, Extrahera, LC-MS/MS, Metanephrine, Metanephrines, Norepinephrine, Normetanephrine, Posters, Urine
This poster describes a method for the simultaneous extraction (using EVOLUTE EXPRESS WCX) and analysis of plasma catecholamines and metanephrines. A highly sensitive, linear and rugged assay was developed, and automated using the Biotage Extrahera sample preparation processing system.
MSACL EU 2016
Tags: 3-methoxytyramine, Catecholamines, Clinical, Dopamine, English, Epinephrine, Extrahera, LC-MS/MS, Metanephrine, Metanephrines, Norepinephrine, Normetanephrine, Plasma, Posters
This poster investigates various parts of the SPE method development process to develop a highly sensitive LC-MS/MS method for analysis of catecholamines (epinephrine, norepinephrine, dopamine).
A number of steps are optimized to improve sensitivity of the
analytes. This includes reducing the buffer strength in the mobile
phase, reducing the buffer strength of washes, careful pH control
throughout the extraction, avoiding an evaporation step and
incorporating IPA in the reconstitution solvent.
Tags: 96-well plate, Catecholamines, Clinical, Dopamine, English, Epinephrine, LC-MS/MS, Norepinephrine, Plasma, Posters
Urine samples are neutralized using 0.5 M ammonium acetate and loaded onto weak cation exchange (WCX) Evolute Express plates (Biotage), preconditioned with methanol and water. Samples are washed with 20 mM ammonium acetate and water before elution in 0.5 % formic acid.
Department of Clinical Chemistry and Immunology, Heart of England NHS Foundation Trust, Birmingham, UK
This poster examines various factors in the development and optimisation of a SPE-LC-MS/MS method for extraction of catecholamines from plasma.
The final method utilizes EVOLUTE EXPRESS WCX plates in either standard SPE or fast Load-wash-elute modes, and incorporates a series of wash steps to optimise analytical sensitivity. Low elution volumes mean no evaporation step is required, and LLOQ is 20mg/mL or lower for each of the analytes.
Tags: 96-well plate, Catecholamines, Clinical, Dopamine, English, Epinephrine, LC-MS/MS, Norepinephrine, Plasma, Posters
EVOLUTE® EXPRESS SPE columns and plates combine sorbent wettability with optimized SPE components, allowing better flow consistency and in many cases eliminating the need for SPE column conditioning; thus simplifying and reducing extraction processing. This poster compares the performance of various EVOLUTE EXPRESS column chemistries using 10-500 mg bed weights, in order to investigate whether sorbent conditioning and equilibration steps are truly required.
Tags: Acidic, Amitriptyline, Atenolol, Benzenesulfonic acid, Bretylium, Clinical, Column, Diethyldithiophosphate (DEDTP), Dimethylthiophosphate (DMTP), English, Extrahera, Forensic, Hexanesulfonic acid, Ketoprofen, LC-MS/MS, Metoprolol, Mianserin, Organophosphate Pesticide, Over the counter pharmaceuticals, Pain Management, Pharma, Pharmaceuticals, Posters, Prednisolone, Procainamide, Quaternary Amines, Quinidine, Salbutamol, Sulindac, Tricyclic Antidepressant, Urine, β-blockers
This poster details current sample preparation strategies for aminoglycoside antibiotics in meat developed in a collaborative effort with Biotage and Tyson Foods, Inc. Springdale Corporate Laboratory to optimize analyte recovery (>70%), minimize analyte suppression and maintain acceptable method precision.
FAST LC-MS 2014
Tags: Aminoglycoside antibiotics, Chicken meat, Column, English, Food, Gentamicin, LC-MS/MS, Neomycin, Posters, Spectinomycin, Streptomycin
This report details a “load, wash, elute” weak cation exchange solid phase extraction procedure amenable to both plasma and urine samples. The extracts are subsequently injected into an LC-MS/MS system. The preliminary sample preparation method was developed at the Biotage US Applications lab (Charlotte, NC).The method was then transferred to Ionics (Bolton, ON, Canada) to facilitate the nmole/L measurements of the selected biomarkers
by laminar flow tandem mass spectrometry. The SPE-LC-ESIMS/ MS method parameters were first optimized using pooled mixed gender plasma. A set of patient samples (n=32) was later supplied by the Mayo Clinic (Rochester, MN) that had previously been analyzed by a validated referee method. The population represented measured values across a range of clinical relevance.
Tags: 96-well plate, Catecholamines, Clinical, English, LC-MS/MS, Metanephrine, Normetanephrine, Plasma, Posters, Urine
This poster focuses on the different strategies that can be employed when using EVOLUTE SPE sorbents (with both single and dual retention mechanisms) to reduce or eliminate residual phospholipids in sample extracts.
Tags: 96-well plate, Clinical, English, Forensic, LC-MS/MS, Pharma, Posters
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
Tags: Biological fluids, English, HPLC, LC-MS/MS, Plasma, Posters
This poster describes the use of EVOLUTE® ABN and EVOLUTE® EXPRESS ABN for the fast and efficient extraction of a diverse mixture of peptides spiked into biological matrices.
Tags: 96-well plate, ACTH (1-24), Angiotensin Fragment 1-7, Angiotensin II,, Clinical, Elafin, Endorphin, English, Glycoprotein myelin, Helicobacter pylori (2-20), LC-MS/MS, Peptides, Pharma, Plasma, Posters, Serum, Vasoactive Intestinal Fragment 1-12
In this poster, an IONICS 3Q 220 triple quadrupole mass spectrometer using a load, wash, elute method protocol with recently advanced solid phase extraction (SPE) sorbent technology (EVOLUTE EXPRESS WCX) was used to perform analysis of the two compounds. As a result low levels of metanephrine and normetanephrine are detectable in plasma with short sample preparation and LC run times. This LC-MS/MS method provides a fast, sensitive, accurate, and reproducible solution for the analysis of ME and NME in plasma.
Tags: 96-well plate, Clinical, English, LC-MS/MS, Metanephrine, Normetanephrine, Plasma, Posters
We detail here the application of new column technology to purify histamine and serotonin (Table 1) in one extraction method with the elimination of the sorbent conditioning and equilibration steps familiar to traditional solid phase extraction (SPE) method protocols prior to measurements by ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-MS/MS). A secondary goal of combining two LC methods into one method was also achieved.
EVOLUTE EXPRESS cation exchange (CX), weak cation exchange (WCX) and acid-base-neutral (ABN) sorbents in a 96 well plate format prior to analysis by UPLC-MS/MS.
Tags: 96-well plate, Biomarker, Clinical, English, Histamine, LC-MS/MS, Mouse plasma, Pharma, Posters, Serotonin
This poster outlines a new method for the extraction of a mixture of peptides from plasma using resin based SPE. Initial method optimization was performed using EVOLUTE ABN before comparison to the streamlined approach without plate conditioning and equilibration using EVOLUTE EXPRESS ABN. Presented at EBF 2012.
EBF, EBF 2012, Peptide, ABN, SPE, Polymer, Polymeric,
Tags: ACTH (1-24), Alpha-Endorphin, Angiotensin Fragment 1-7, Angiotensin II,, Elafin, Helicobacter pylori (2-20), LC-MS/MS, Myelin Glycoprotein fragment (35-55) rat, Peptides, Pharma, Plasma, Posters, Vasoactive Intestinal Fragment 1-12
This technical note provides guidelines for the extraction of quaternary amines and strongly basic analytes from aqueous samples using EVOLUTE WCX (Method A). A method optimized for extraction of a wide range of basic compounds, allowing elution under mildly acidic conditions (Method B) is also detailed. This approach is recommended for the extraction of basic compounds when basic elution conditions are unsuitable, or acidic elution conditions are preferred due to LC-MS/MS compatibility. The procedures are described on page 1–2, with processing and optimisation guidelines on page 3. An example application showing the extraction of quaternary amines from urine and plasma illustrates the use of EVOLUTE WCX (Chlormequat, Neostigmine, Bretylium, Difenzoquat) for clean-up of biological fluid samples.
BASIC, BIOTAGE, EVOLUTE, OASIS, SPE, POLYMER, SOLID PHASE EXTRACTION
Tags: 96-well plate, Aqueous, Basic drugs, Biological fluids, Bretylium, Chlormequat, Column, Difenzoquat, Drugs of Abuse, LC-MS/MS, Neostigmine, Plasma, Product Notes, Quaternary Amines, Serum, Urine, Water
EVOLUTE® WCX is a weak cation exchange mixed-mode
resin-based SPE sorbent that extracts a range of strongly
basic analytes and quaternary amines from biological fluids
and other aqueous matrices using a generic procedure
which minimizes method development time. EVOLUTE WCX
removes matrix components such as proteins, salts, nonionizable
interferences and phospholipids, delivering
cleaner extracts with reproducible recoveries for accurate
Tags: Product Notes
Thursday 9th March 2017 11.00am Central European Time (10.00am Greenwich Mean Time) then repeated at 1.00pm US Eastern Time (10.00am US Pacific Time) REGISTER NOW » T...17 February 2017
This application note describes a mixed-mode weak cation exchange SPE protocol for the extraction of catecholamines and metanephrine metabolites from urine prior to LC-MS/MS detection. The...06 December 2016
Could there be a sorbent for versatile sample preparation that doesn't need conditioning and equilibration? Actually, Biotage has developed five such sorbents in the EVOLUTE® EXPRESS family, an...29 September 2016
EVOLUTE® EXPRESS WCX 96 well plates can be used to extract catecholamines and metanephrines from pooled human plasma in a highly sensitive, linear and rugged assay. This was demonstrated in a&n...20 April 2016
An ISO accredited method for analysis of urinary free catecholamines by mass spectrometry was presented in a poster at MSACL 2016 in Palm Springs, USA. The method, now ...22 October 2015
A new application note from Biotage labs describes an optimized method for extraction of three catecholamines from human plasma. The extraction method utilizes the EVOLUTE® EXPRE...15 October 2015
A new application note from Biotage describes an optimized method for extraction of the catecholamines dopamine, epinephrine and norepinephrine from human plasma. The extraction method utilizes the...15 September 2015
Catecholamines are biomarkers used for the detection of diseases such as: hypertension, pheochromocytoma and neuroblastoma. The main target analytes are dopamine epinephrine and norepinephrine, whi...30 March 2015
Three case studies were presented by Biotage at MSACL 2015 in San Diego, CA. The studies refer to various analyte extraction methods prior to GC/MS and LC/MS.27 March 2015
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...05 November 2014
With expert support from Tyson Foods, Biotage labs has co-developed an improved method for analyzing residues of aminoglycosides in meat. This study evaluates the use of EVOLUTE® WCX s...23 October 2014
A simplified method for screening metanephrine and normetanephrine proves as effective as the current gold standard. It is anticipated that this time-saving and sensitive SPE-LC-ESI-MS/MS...29 July 2014
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...10 July 2014
Eight new posters by Biotage Scientists and collaborating groups have been presented at ASMS 2014, Baltimore, MD, USA. P81 A Reduced Workflow Method For The Extraction Of Vitamin B7 (bi...30 June 2014
Biotage is attending EPRW (European Pesticides and Residues Workshop) 2014 30th June - 3rd July 2014, Dublin, Ireland Read more at conference website http://eprw2014.com/20 May 2014
Four new posters by Biotage R&D and Application Scientists have been presented this week at HPLC 2014, New Orleans, LA, USA. The studies focus on clean-up of biofluids using different sa...01 April 2014
Biotage is attending the Annual Meeting of the German Society for Experimental and Clinical Pharmacology and Toxicology (DGPT) 1st - 3rd April 2014, Hannover, Germany Read more at the conference ...11 January 2014
Biotage is attending the 22nd Congress French Toxicology Society19th Meeting Society of Hair Testing Annual Meeting Belgian and Luxembourg Toxicological Society 11th-13th June 2014, Bordeuax, Fran...28 August 2012
EVOLUTE EXPRESS 96-Well SPE EVOLUTE EXPRESS® represents a leap forward in high throughput solid phase extraction. The EVOLUTE EXPRESS range of 96-well SPE plates combine powerful EVOLUTE sorbe...28 August 2012
Uppsala, Sweden, 28th August, 2012 Biotage® (STO: BIOT), a leading global supplier of solutions and technology for analytical and medicinal chemistry, introduced EVOLUTE® EXPRESS, a novel ...18 April 2011
Uppsala, Sweden – Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, today announced the further expansion of its successful family of sample preparat...16 December 2010
Biotage Announces an Easy, Cost-Effective Approach to the Analysis of Ethyl Glucuronide (EtG) in Urine for Clinical and Forensic Toxicology Laboratories Uppsala, Sweden – Biotage®...23 February 2010
Uppsala, Sweden, March 2, 2010– Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, today announced the further expansion of its successful family of p...
Is there an expiry (‘best before’) date for ISOLUTE and EVOLUTE sample preparation products?
There is no fixed expiry date for these products.
All ISOLUTE and EVOLUTE products are packaged in inner metal laminate barrier bags, which provide an effective barrier to oxygen and moisture penetration. Recommended storage conditions are standard (see below).
We have no indications that the performance of non-bonded phase SPE sorbents is affected by long term storage, under recommended conditions.
We have no indications that the performance of bonded phase SPE sorbents is affected by long term storage, under recommended conditions.Browse more questions and answers >
|Part No.||Description||Product type||Pack Size||Price|
|602-0001-AXG||EVOLUTE EXPRESS WCX 10 mg / 1 mL (Tabless)||Consumables||100||Log in for price|
|602-0003-AXG||EVOLUTE EXPRESS WCX 30 mg / 1 mL (Tabless)||Consumables||100||Log in for price|
|602-0010-PX01||EVOLUTE EXPRESS WCX 10 mg Fixed Well plate||Consumables||1||Log in for price|
|602-0030-PX01||EVOLUTE EXPRESS WCX 30 mg Fixed Well plate||Consumables||1||Log in for price|
|612-0010-BXG||EVOLUTE EXPRESS WCX 100 mg / 3 mL Tabless||Consumables||50||Log in for price|
|612-0015-CXG||EVOLUTE EXPRESS WCX 150 mg /6 mL (Tabless)||Consumables||30||Log in for price|
|612-0050-CXG||EVOLUTE EXPRESS WCX 500 mg /6 mL (Tabless)||Consumables||30||Log in for price|
|650-0010-PX01||EVOLUTE EXPRESS SORBENT SELECTION 10 mg Fixed Well plate||Consumables||1||Log in for price|
|650-0030-PX01||EVOLUTE EXPRESS SORBENT SELECTION 30 mg Fixed Well plate||Consumables||1||Log in for price|
|Part No.||Description||Product type||Pack Size||Price|
|121-9620||VacMaster-96 Tabless 1 mL Column Holder||Accessories||1||Log in for price|
|PPM-A96-CH||PRESSURE+ 96 Column Holder 96||Accessories||1||Log in for price|