|Chemical description||Primary/secondary amine modified polystyrene-divinylbenzene incorporating non-ionizable hydroxyl groups|
|Sorbent type||Mixed-mode non-polar/weak anion exchange|
|Exchange capacity||0.3 mmol/g|
|Average particle size||30 µm, 50 µm|
|Pore diameter||40 Å|
This application note describes a simple, effective protocol for the extraction of methylmalonic acid (MMA) from serum using EVOLUTE® EXPRESS WAX solid phase extraction plates, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts.
The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags: 96-well plate, Acidic, Application Notes, Clinical, English, Extrahera, LC-MS/MS, MMA (Methylmalonic acid), Methylmalonic acid (MMA), Serum
This application note describes a Biotage polymer-based mixed-mode weak anion exchange SPE protocol for the extraction of a range of polyfluorinated (PFA) compounds in water prior to LC-MS/MS detection.
High (>80%) reproducible analyte recoveries, with excellent linearity to LOQs of 0.1 ng/L or below are achieved.
Tags: Application Notes, Column, English, Environmental, LC-MS/MS, Perfluorobutanesulfonic acid (PFBuS), Perfluorodecanoic acid (PFDA), Perfluoroheptanoic acid (PFHpA), Perfluorohexanesulfonic acid (PFHxS), Perfluorohexanoic acid (PFHxA), Perfluorononanoic acid (PFNA), Perfluorooctanesulfonic acid (PFOS), Perfluorooctanoic acid (PFOA), Persistant organic pollutants (POP), Polyfluorinated acids, Water
This method describes the use of EVOLUTE WAX plates and columns to extract a suite of organophosphate pesticide metabolites including dimethylphosphate (DMP), dimethylthiophosphate (DMTP), diethylphosphate (DEP), diethylthiophosphate (DETP), dimethyldithiophosphate (DMDTP) and diethyldithiophosphate (DEDTP) from human urine. The method demonstrates extraction of these small polar metabolites at urine concentrations ranging from 40 ng/mL to 400 ng/mL.
resin, polymer, EVOLUTE,
Tags: Application Notes, Clinical, Column, Diethyldithiophosphate (DEDTP), Diethylphosphate (DEP), Diethylthiophosphate (DETP), Dimethyldithiophosphate (DMDTP), Dimethylphosphate (DMP), Dimethylthiophosphate (DMTP), LC-MS/MS, Organophosphate Pesticide, Urine
In this catalog product information is displayed with easy-to-follow application details and supporting documentation.
Ordering information is found following each product listing.
EVOLUTE® EXPRESS Solid Phase Extraction products
from Biotage are designed for all your sample
preparation challenges. Whether you are working in the field of bio-analysis during drug development, clinical or forensic
toxicology, or with the diversity of samples in food
safety and environmental applications, the tools and
methods used for sample preparation have a clear
impact on productivity. This user guide contains information about choosing the correct EVOLUTE EXPRESS product for your application and optimizing your methodology for best results.
Tags: 96-well plate, Clinical, Column, English, Environmental, Food, Forensic, Manuals & User Guides, On-line SPE, Pharma
Whether working in the field of bio-analysis in
drug development, clinical or forensic analysis, or
with the diversity of samples in food safety and
environmental applications, the tools and
methods used for sample preparation have a
clear impact on productivity during method
development, validation and routine analysis.
EVOLUTE SPE sorbents and extraction
methodologies have been developed to extract a
wide range of analytes while reducing or
eliminating matrix components that can cause ion
suppression in LC-MS analysis.
Tags: Clinical, Environmental, Food, Forensic, Manuals & User Guides, Pharma
This poster summarizes various sample preparation strategies for the
extraction of MMA from serum without the necessity for derivatization, prior to LC-MS/MS analysis. A range of sample preparation techniques of varying complexity were evaluated: protein precipitation, phospholipid depletion, supported liquid extraction and solid phase extraction using both silica and polymer-based mixed-mode anion exchange chemistries.
Method performance was evaluated for evaporative effects, assay recovery, ion suppression and phospholipid removal.
Tags: 96-well plate, Acidic, Clinical, English, LC-MS/MS, MMA (Methylmalonic acid), Methylmalonic acid (MMA), Posters, Serum
This poster examines various factors in the development and optimisation of a SPE-LC-MS/MS method for extraction of catecholamines from plasma.
The final method utilizes EVOLUTE EXPRESS WCX plates in either standard SPE or fast Load-wash-elute modes, and incorporates a series of wash steps to optimise analytical sensitivity. Low elution volumes mean no evaporation step is required, and LLOQ is 20mg/mL or lower for each of the analytes.
Tags: 96-well plate, Catecholamines, Clinical, Dopamine, English, Epinephrine, LC-MS/MS, Norepinephrine, Plasma, Posters
EVOLUTE® EXPRESS SPE columns and plates combine sorbent wettability with optimized SPE components, allowing better flow consistency and in many cases eliminating the need for SPE column conditioning; thus simplifying and reducing extraction processing. This poster compares the performance of various EVOLUTE EXPRESS column chemistries using 10-500 mg bed weights, in order to investigate whether sorbent conditioning and equilibration steps are truly required.
Tags: Acidic, Amitriptyline, Atenolol, Benzenesulfonic acid, Bretylium, Clinical, Column, Diethyldithiophosphate (DEDTP), Dimethylthiophosphate (DMTP), English, Extrahera, Forensic, Hexanesulfonic acid, Ketoprofen, LC-MS/MS, Metoprolol, Mianserin, Organophosphate Pesticide, Over the counter pharmaceuticals, Pain Management, Pharma, Pharmaceuticals, Posters, Prednisolone, Procainamide, Quaternary Amines, Quinidine, Salbutamol, Sulindac, Tricyclic Antidepressant, Urine, β-blockers
This poster focuses on the different strategies that can be employed when using EVOLUTE SPE sorbents (with both single and dual retention mechanisms) to reduce or eliminate residual phospholipids in sample extracts.
Tags: 96-well plate, Clinical, English, Forensic, LC-MS/MS, Pharma, Posters
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
Tags: Biological fluids, English, HPLC, LC-MS/MS, Plasma, Posters
This poster describes the use of EVOLUTE® ABN and EVOLUTE® EXPRESS ABN for the fast and efficient extraction of a diverse mixture of peptides spiked into biological matrices.
Tags: 96-well plate, ACTH (1-24), Angiotensin Fragment 1-7, Angiotensin II,, Clinical, Elafin, Endorphin, English, Glycoprotein myelin, Helicobacter pylori (2-20), LC-MS/MS, Peptides, Pharma, Plasma, Posters, Serum, Vasoactive Intestinal Fragment 1-12
This poster outlines a new method for the extraction of a mixture of peptides from plasma using resin based SPE. Initial method optimization was performed using EVOLUTE ABN before comparison to the streamlined approach without plate conditioning and equilibration using EVOLUTE EXPRESS ABN. Presented at EBF 2012.
EBF, EBF 2012, Peptide, ABN, SPE, Polymer, Polymeric,
Tags: ACTH (1-24), Alpha-Endorphin, Angiotensin Fragment 1-7, Angiotensin II,, Elafin, Helicobacter pylori (2-20), LC-MS/MS, Myelin Glycoprotein fragment (35-55) rat, Peptides, Pharma, Plasma, Posters, Vasoactive Intestinal Fragment 1-12
This poster will demonstrate SPE sample preparation strategies for the simultaneous extraction of both ethyl glucuronide and ethyl sulphate from urine, allowing cleaner extraction protocols compared to traditional dilute and shoot approaches.
EtG, EtS, Urine, Poster, SOFT, Forensic, UPLC, LC-MS/MS, EVOLUTE, AX, WAX
Tags: 96-well plate, Alcohol metabolite, Column, Ethyl Sulfate (EtS), Ethyl glucuronide (EtG), Forensic, LC-MS/MS, Posters, Urine
This technical note provides guidelines for the extraction of strongly acidic compounds from aqueous samples using EVOLUTE WAX. The procedure is described on pages 1, with processing and optimization guidelines on page 2. An example application showing the extraction of pesticide metabolites from urine illustrates the use of EVOLUTE WAX (dimethylthiophosphate (DMTP), diethylphosphate (DEP), hexanesulfonic acid (HSA), benzenesulfonic acid (BSA)) for clean-up of biological fluid samples.
POLYMER, ACIDIC, WEAK ANION EXCHANGER, EVOLUTE, SPE, SAMPLE PREP, SAMPLE PREPARATION, OASIS
Tags: 96-well plate, Acidic drugs, Aqueous, Biological fluids, Column, Diethylphosphate (DEP), Dimethylthiophosphate (DMTP), Drugs of Abuse, LC-MS/MS, Organophosphate Pesticide, Plasma, Product Notes, Serum, Urine
EVOLUTE® WAX mixed mode polymer-based SPE sorbent
extracts a range of acidic analytes from biological fluids and
other aqueous matrices using a generic procedure which
minimizes method development time. EVOLUTE WAX removes
matrix components such as proteins, salts, non-ionisable
interferences and phospholipids, delivering cleaner extracts
with reproducible recoveries for accurate quantitation.
Tags: Biological fluids, Product Notes
Thursday 9th March 2017 11.00am Central European Time (10.00am Greenwich Mean Time) then repeated at 1.00pm US Eastern Time (10.00am US Pacific Time) REGISTER NOW » T...06 December 2016
Could there be a sorbent for versatile sample preparation that doesn't need conditioning and equilibration? Actually, Biotage has developed five such sorbents in the EVOLUTE® EXPRESS family, an...02 February 2016
Five new application notes from Biotage labs provide a suite of methods for effective extraction of methylmalonic acid from serum using modern sample preparation techniques and materials from ...15 October 2015
A new application note from Biotage describes an optimized method for extraction of the catecholamines dopamine, epinephrine and norepinephrine from human plasma. The extraction method utilizes the...27 March 2015
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...25 March 2015
This application note demonstrates the use of EVOLUTE® WAX solid phase extraction columns for the extraction of a range of polyfluorinated (PFA) compounds from water samples. The ...29 July 2014
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...10 July 2014
Eight new posters by Biotage Scientists and collaborating groups have been presented at ASMS 2014, Baltimore, MD, USA. P81 A Reduced Workflow Method For The Extraction Of Vitamin B7 (bi...30 June 2014
Biotage is attending EPRW (European Pesticides and Residues Workshop) 2014 30th June - 3rd July 2014, Dublin, Ireland Read more at conference website http://eprw2014.com/20 May 2014
Four new posters by Biotage R&D and Application Scientists have been presented this week at HPLC 2014, New Orleans, LA, USA. The studies focus on clean-up of biofluids using different sa...01 April 2014
Biotage is attending the Annual Meeting of the German Society for Experimental and Clinical Pharmacology and Toxicology (DGPT) 1st - 3rd April 2014, Hannover, Germany Read more at the conference ...11 January 2014
Biotage is attending the 22nd Congress French Toxicology Society19th Meeting Society of Hair Testing Annual Meeting Belgian and Luxembourg Toxicological Society 11th-13th June 2014, Bordeuax, Fran...28 August 2012
Uppsala, Sweden, 28th August, 2012 Biotage® (STO: BIOT), a leading global supplier of solutions and technology for analytical and medicinal chemistry, introduced EVOLUTE® EXPRESS, a novel ...28 August 2012
EVOLUTE EXPRESS 96-Well SPE EVOLUTE EXPRESS® represents a leap forward in high throughput solid phase extraction. The EVOLUTE EXPRESS range of 96-well SPE plates combine powerful EVOLUTE sorbe...18 April 2011
Uppsala, Sweden – Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, today announced the further expansion of its successful family of sample preparat...16 December 2010
Biotage Announces an Easy, Cost-Effective Approach to the Analysis of Ethyl Glucuronide (EtG) in Urine for Clinical and Forensic Toxicology Laboratories Uppsala, Sweden – Biotage®...23 February 2010
Uppsala, Sweden, March 2, 2010– Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, today announced the further expansion of its successful family of p...
Is there an expiry (‘best before’) date for ISOLUTE and EVOLUTE sample preparation products?
There is no fixed expiry date for these products.
All ISOLUTE and EVOLUTE products are packaged in inner metal laminate barrier bags, which provide an effective barrier to oxygen and moisture penetration. Recommended storage conditions are standard (see below).
We have no indications that the performance of non-bonded phase SPE sorbents is affected by long term storage, under recommended conditions.
We have no indications that the performance of bonded phase SPE sorbents is affected by long term storage, under recommended conditions.Browse more questions and answers >
|Part No.||Description||Product type||Pack Size||Price|
|604-0001-AXG||EVOLUTE EXPRESS WAX 10 mg / 1 mL (Tabless)||Consumables||100||Log in for price|
|604-0003-AXG||EVOLUTE EXPRESS WAX 30 mg / 1 mL (Tabless)||Consumables||100||Log in for price|
|604-0010-PX01||EVOLUTE EXPRESS WAX 10 mg Fixed Well plate||Consumables||1||Log in for price|
|604-0030-PX01||EVOLUTE EXPRESS WAX 30 mg Fixed Well plate||Consumables||1||Log in for price|
|614-0006-BXG||EVOLUTE EXPRESS WAX 60 mg / 3 mL (Tabless)||Consumables||50||Log in for price|
|614-0010-BXG||EVOLUTE EXPRESS WAX 100 mg / 3 mL Tabless||Consumables||50||Log in for price|
|614-0015-CXG||EVOLUTE EXPRESS WAX 150 mg /6 mL (Tabless)||Consumables||30||Log in for price|
|614-0050-CXG||EVOLUTE EXPRESS WAX 500 mg /6 mL (Tabless)||Consumables||30||Log in for price|
|650-0010-PX01||EVOLUTE EXPRESS SORBENT SELECTION 10 mg Fixed Well plate||Consumables||1||Log in for price|
|650-0030-PX01||EVOLUTE EXPRESS SORBENT SELECTION 30 mg Fixed Well plate||Consumables||1||Log in for price|
|Part No.||Description||Product type||Pack Size||Price|
|121-9620||VacMaster-96 Tabless 1 mL Column Holder||Accessories||1||Log in for price|
|PPM-A96-CH||PRESSURE+ 96 Column Holder 96||Accessories||1||Log in for price|