EVOLUTE® WAX combines non-polar and weak anion exchange functionality for extraction of strongly acidic analytes from biological fluids and other aqueous samples through a dual, mixed-mode mechanism. This provides additional selectivity and clean-up for complex samples, with salts, phospholipids and proteins eliminated from extracts. Available in a variety of formats, including modular and 96-well plates and SPE columns, and two different particle sizes (30 µm and 50 µm) depending on application requirements.
The sorbent consists of the EVOLUTE backbone, surface modified with primary/secondary amine groups.
Figure 1. Structure of EVOLUTE® WAX sorbent
EVOLUTE® WAX sorbent is available in EVOLUTE® EXPRESS SPE columns and 96-well plate formats for use with fast Load-Wash-Elute procedures. Eliminate traditional conditioning and equilibration and increase throughput without compromising performance.
High Selectivity Provides Ultra-clean Extracts
EVOLUTE sorbents have a narrow pore diameter, tailored to eliminate co-extraction of proteins from biofluids. Removal of proteins during sample preparation avoids transfer in to the analytical system, reducing matrix effects in LC-MS/MS, and avoiding contamination of the analytical column. This minimises the need for frequent backflushing and guard columns replacement.
Using EVOLUTE WAX, interferences including proteins, salts, non-ionisable molecules and phospholipids are removed during the interference elution step, ensuring extremely clean extracts (figure 2).
Figure 2. The combination of optimized pore structure and additional interference elution steps using mixed-mode sorbent chemistry guarantees extremely clean extracts.
High Analyte Recoveries
EVOLUTE® WAX uses a generic procedure for extraction of strongly acidic analytes in aqueous matrices. The dual retention mechanism retains strong acids through a combination of non-polar and weak anion exchange interactions. The simple wash steps in the generic method remove >98% of phospholipids from plasma samples.
The combination of robust polymer chemistry, uniform coverage of secondary amine groups and optimum exchange capacity provide greater than 85 % recovery for a wide range of strongly acidic analytes.
See the EVOLUTE User Guide for a wealth of method optimisation information.
Figure 2. Recovery of strongly acidic analytes from urine samples using EVOLUTE WAX fixed well plate. Analytes: dimethylthiophosphate (DMTP), diethylphosphate (DEP), hexanesulfonic acid (HSA), benzenesulfonic acid (BSA).
Excellent Flow Characteristics
Narrow particle size distribution provides consistent flow characteristics for a variety of sample matrices. Optimized packing techniques eliminate channelling, which can lead to analyte breakthrough.
Even viscous biofluids can be loaded onto EVOLUTE EXPRESS WAX products without the need for conditioning and equilibration using fast load-wash-elute SPE.
The surface characteristics of each batch of EVOLUTE® WAX are quality controlled and tested to ensure consistency.
- High, reproducible recoveries for strongly acidic analytes
- Eliminate salts, phospholipids and proteins from biofluid extracts, and minimise ion suppression
- Fast simple generic methodology
- Available in EVOLUTE EXPRESS SPE columns and plates for fast Load-Wash-Elute procedures