This guide describes simple practical strategies, hints and tips designed to mitigate or avoid cross-well contamination or ‘cross talk’ in high throughput 96-well based sample preparation techniques.
Tags: 96-well plate, Brochures, Clinical, English, Forensic, Pharma
Biotage offers the widest range of solvent evaporation systems available. This guide presents the most suitable evaporation system for your research requirements, and answers common questions regarding service, maintenance and applications.
Keywords: turbovap, turbo vap, v10, v-10, spedry, spe dry, evaporation, concentrat
Tags: Brochures, English
Biotage® ACT Plate Adapter (patent pending) is designed to reduce or eliminate the occurrence of well-to-well cross contamination (cross talk) during extract evaporation, and fits on top of the 96-well collection plate during evaporation.
Tags: Manuals & User Guides
This poster compares the performance of manual processing to a novel automated sample preparation system prior to GC/MS or LC-MS/MS analysis in forensic toxicology applications. Emphasis is placed on the potential for 96-well cross contamination and strategies for its elimination.
Tags: 2-Hydroxyethylflurazepam, 2-OH-Et-Flurazepam, 6-acetylmorphine (MAM), 7-Aminoclonazepam, 7-Aminoflunitrazepam, 96-well plate, Alprazolam, Amphetamine, Amphetamines, Benzodiazepines, Bromazepam, Buprenorphine, Clinical, Clonazepam, Codeine, Column, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Estazolam, Extrahera, Fentanyl, Flunitrazepam, Flurazepam, Forensic, GC-MS, Hydrocodone, Hydromorphone, Ketamine, LC-MS/MS, Lorazepam, MDA, MDEA, MDMA, Mephedrone, Methadone, Methamphetamine, Midazolam, Morphine, Nitrazepam, Norbuprenorphine, Nordiazepam, Norfentanyl, Norketamine, Opiates, Opiates, Oxazepam, Oxycodone, Oxymorphone, Phencyclidine (PCP), Posters, Temazepam, Triazolam, Urine, Whole blood, Zaleplone, Zolpidem, Zopiclone, z-drugs, α-OH Triazolam, α-OH alprazolam
This poster demonstrates a rapid and reliable sample preparation method using Supported Liquid Extraction (ISOLUTE SLE+) to extract a suite of 30 hormone analytes from a hydrolyzed urine matrix. Single injection analysis by LC-MS/MS shows that matrix effects are eliminated by the ISOLUTE SLE+ protocol and that analyte recovery and sensitivity have excellent clinical utility.
Tags: 11-deoxycortisol, 11-keto-androsterone, 11-oxoetiocholanolone, 11-β-hydroxyandrosterone, 11β-hydroxyetiocholanolone, 2-OH-estrone, 2-methoxyestrone, 3α-androstanediol, 3β-androstanediol, 4-OH-estrone, 4-methoxyestrone, 96-well plate, Allo-THB, Androsterone, Clinical, Corticosterone, DHEA (dehydroepiandrosterone, Dehydroepiandrosterone (DHEA), English, Etiocholanolone, Extrahera, Hydrolysed urine, Posters, Steroid Hormones, Steroid metabolite, THB, THF, Testosterone, α-THF
This poster discusses various stages of the sample preparation process to determine and assess the potential for cross contamination and present approaches to minimize and or eliminate the effect.
Tags: 96-well plate, Clinical, English, Forensic, LC-MS/MS, Pharma, Posters
In all areas of analytical laboratory testing it is vital to ensure proper quality measures are in place to reduce or eliminate cross contamination between samples, which could result in false positive and/or false negative results. In many cases sample carryover in the LC/MS system is checked early on in the method development process. However, one area that can often be overlooked the sample preparation stage. This involves all aspects including pipetting, sample transfer, extraction protocol, evaporation and mixing steps. This poster examines various stages of the sample preparation process to determine the potential for cross contamination and present approaches to minimize and or eliminate the effect. This is demonstrated via a series of dye experiments combined with analyte testing using LC-MS/MS.
Tags: 96-well plate, Clinical, English, Forensic, LC-MS/MS, Opiates, Pharma, Posters
This product note details technical and ordering information for the Biotage® ACT Plate Adapter, a novel accessory for prevention of cross talk during evaporation.
Tags: 96-well plate, Clinical, English, Forensic, Pharma, Product Notes
Recovery profiles from manual and automated supported liquid extraction protocols were put side by side in a study on drugs of abuse in blood and urine matrices. Data reliability in forens...16 July 2015
Uppsala, Sweden, 15th July 2015 – Biotage® (STO: BIOT), a leading global supplier of solutions and technology for analytical and medicinal chemistry, announces the launch of Biotage® ...
Designed for high throughput laboratories, the Biotage® SPE Dry 96 and Biotage®SPE Dry 96 Dual Sample Concentrator Systems provide efficient solvent evaporation in microplate format and are compatible with 24-, 96- and 384-well collection plates. Heated gas flow from above and below the collection plate ensures efficient solvent evaporation.
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