Item added to cart
Shopping Cart (Login required)
Sample Preparation Organic Synthesis Peptide Chemistry Flash Purification Work-up Products Evaporation Process Development Industrial Products Service & Support

Biotage® ACT Plate Adapter

Protect Your Samples from Cross Contamination During Evaporation

414355SP
414355SP ACT Plate Adapter in situ needles up ACT Plate adapter in situ needles down 414355SP

Biotage® ACT (Anti Cross Talk) Plate Adapter is a novel (patent pending) solution to the phenomenon of hot spot cross contamination, or cross talk, during evaporation.

Easy to incorporate into any 96-well based assay, this essential evaporation accessory can save the cost and time of sample re-runs.

The simple but effective design prevents cross talk on evaporation, decreasing the risks of falsely elevated results. Its high specification anodized aluminum construction allows it to be reused, and it is easily cleaned between runs to prevent any potential carry over.

Biotage ACT Plate Adapter is designed for use with square well colection plates, and is compatible with 'straight needle' 96-well plate evaporators such as the Biotage SPE Dry 96 and SPE Dry 96 Dual Sample Concentrator Systems.

Download the Product Note

Find out more about potential causes of cross talk in 96-well sample preparation, and how to avoid them, in our comprehensive guide

Find out more about LC-MS/MS troubleshooting, and approaches to solving the problem of false positives in the context of a high throughput clinical laboratory in this presentation (scroll to 'LC-MS/MS Troubleshooting at a Large Reference Lab' by Alan Rockwood, PhD, courtesy of MSACL.org) 

 

ACCESSORIES

Part No. Description Product type Pack Size Price
414355SP Biotage® ACT Plate Adapter Accessories 1 Log in for price

 

The Problem 

There are many potential causes of sample cross contamination in any clinical or forensic toxicology assay, particularly with the increasing use of the high throughput, low volume 96-well pate format for sample preparation. One recently identified area of concern is during evaporation.

During the evaporation stage, a proportional amount of analytes present in an extracted sample can contaminate surrounding wells by being carried with the volatilized solvent. When analyte concentration in the “hotspot” well is considerably higher than the analyte concentration in the surrounding wells the potential exists for extraneous analyte signal in adjacent and/or surrounding wells, thus leading to false positives which could pose severe consequences in any assay.

 

Mechanism for ‘hot spot’ cross talk during evaporation, resulting in carry-over from high-concentration sample wells to low-concentration wells, which show up as false positives.

The Solution 

Biotage® ACT Plate Adapter fits on top of the 96-well collection plate during evaporation. Evaporation gas and solvent vapours are directed away from sample tubes, eddies are reduced, and the adapter reduces the open surface area of the top of the wells, lessening the likelihood that material can re-enter and contaminate adjacent wells.