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Flexibility and Choice for the Modern Day Labs
Biotage was the first company to pre-pack flash chromatography cartridges, and continues to be the innovation leader with four superior designs: Biotage® SNAP, Biotage® SNAP Ultra, Biotage ZIP® and Biotage ZIP® Sphere.
Biotage flash cartridges for purification are manufactured from polypropylene and polyethylene that meet or exceed the extractable requirements in US 21 CFR 177.1520 and are packed using proprietary methods that strictly adhere to ISO 9001:2000 quality standards. Cartridge sizes range from 2 g to 40 kg. Biotage cartridges undergo a stringent series of tests including shock resistance and chromatography testing for maximum quality. SNAP cartridges are laser-etched with a unique lot number to ensure traceability.
Biotage Flash purification
Flash purification is a technique developed by Professor W.C. Still that uses a column or cartridge filled with an insoluble solid support (stationary phase) and elution solvent mixture (mobile phase) to separate and purify a mixture of organic compounds. The stationary phase and the mobile phase typically have very different polarities, which work in tandem to separate compound mixtures. The separated molecules can then be collected in a purified state for use in a subsequent synthesis or as a final product. In 1994, Biotage was the first to develop and introduce pre-packed cartridges for flash purification. A broad selection of distinct cartridge styles now enable professionals to choose the cartridge which best suits the purification need and purification system:
- Biotage® SNAP
- Biotage® SNAP Ultra
- Biotage® SNAP XL
- Biotage Zip®
- Biotage Zip® Sphere
- Biotage® Flash 75
- Biotage® Flash 150
- Biotage® Flash 400
All Biotage cartridges are designed to meet the requirements of HPFC (high-performance flash chromatography) systems including Biotage Isolera™ Prime, Isolera™ One, Four and LS, Biotage SP1, Biotage SP4, and Biotage FlashMaster. Automated cartridge-packing techniques ensure efficiently packed Biotage flash cartridges minimize performance variability. Cartridge sizes range from 5 g to 40+ kg for purification scales ranging from milligrams to kilograms. Manufactured from polypropylene to meet the extractable requirements in US 21 CFR 177.1520 and packed using proprietary methods that strictly adhere to ISO 9001:2000 quality standards, Biotage cartridges also undergo QC testing and production control. Each Biotage SNAP cartridge is laser-etched with a unique lot number to ensure traceability.
PRODUCT DESCRIPTIONS
Reversed-Phase Flash Purification
Normal-phase flash chromatography1 has been widely adopted as the method of choice for separation of product mixtures and reaction by-products. One of the most significant developments in this area concerns the practical separation of polar molecules. Reversed-phase purification is a modification of normalphase chromatography that provides an efficient mechanism for the separation of polar compounds.
APPLICATION NOTES
Biotage Flash Cartridge User Guide - The Definitive Cartridge User Guide
The definitive guide to flash chromatography. This document presents in detail the broad selection of ways that materials for purification can be loaded onto a flash cartridge.
Method Development in Flash Purification
Flash purification involves a simple liquid chromatography technique » Method development uses TLC as a way of deciding the parameters for the separation » Isocratic separations are easiest to develop, but gradient separations are more powerful » Software in the Isolera helps with conversion of an isocratic separation to a gradient » It is possible with the Spektra software to run step gradients » Loading options are dependent on the column type » SNAP offers the most flexibility » Care must be taken to choose the best loading option to get good purifications
OTHER
Why Reusing Flash Cartridges is Bad Science
To save money on consumables, many chemists choose to reuse silica flash cartridges. This is true but risks purification results because chromatographic separation performance will change from run to run which reduces purification quality, especially in normal phase systems. Regardless of the cartridge brand used, repeated use of silica flash cartridges results in loss of compound resolution and therefore fraction purity.
Flash Purification Consumables - A Guide to Cartridges & Method Development
Flash purification is a separation technique developed in 1978 by Professor W.C. Still that uses a stationary phase (a column or cartridge filled with an insoluble solid support) and a mobile phase (elution solvent mixture) to separate and purify a mixture of organic compounds.
Organic Chemistry - Products from Start to Finish
Biotage has developed tools for every step of the organic process, with the entire workflow in mind. This dedicated suite of products vastly expands the range of options in order to truly accelerate discoveries of new molecules for future innovations.
Flash Chromatography: Using UV Detection with UV Absorbing Mobile Phases
The UV absorption spectrum of some solvents overlaps with the product they dissolve, meaning that fraction collection processes cannot distinguish between solvent and product. Luckily, there is technology that solves this problem.
DMSO and DMF as Injection Solvents
DMSO and DMF are suitable injection solvents for reversed-phase flash purification. DMSO shows it can be loaded in larger volumes (up to 0.05 mL/g of C18 media or 3.5% of a column volume) without affecting chromatographic separations or carrying compounds with it.
Impact of Injection Solvent Choice on Peak Shape and Resolution in Reversed-phase Flash Chromatography
Reversed-phase chromatography is typically used when you need to separate several milligrams of relatively polar compounds that either are not soluble in normal-phase solvents or are not compatible with bare silica because they react, stick, or both. If you are currently using reversed-phase at preparative scale, such as flash chromatography, you know the mobile phase limitations – water with either methanol, acetonitrile, or THF. As with normal-phase flash chromatography, when it comes time to purify you want your crude sample fully solubilized in the weakest possible solvent at the highest possible concentration. ACS 2016
Options Available for Green Flash Chromatography
For those chemists performing organic synthesis, reaction mixture purification by flash column chromatography is an integral and necessary part of the synthesis process. However, flash chromatography consumes large volumes of solvent which either needs to be recycled or disposed. ACS 2016.
Greener reversed-phase Flash Chromatography Using Acetone Instead of Acetonitrile
As reversed-phase flash chromatography gains traction in medicinal chemistry labs the need to monitor its cost and safety are becoming more important. Commonly used reversed-phase solvents typically include water with an organic solvent such as methanol or acetonitrile – each have advantages and disadvantages.
Acetonitrile as a Replacement for Methanol in Normal-phase Flash Chromatography
When it comes to polar organic compound purification, many chemists turn to normal-phase flash chromatography often utilizing dichloromethane and methanol as the eluting solvents. While this can work, it often can be challenging to optimize due to methanol’s high polarity and protic chemistry.
Strategies for Improving Flash Chromatography Efficiency
For most organic and natural product chemists flash chromatography is a necessary part of their research. As such, many chemists need quick isolation of at least one desired component from a crude mixture in relatively high yield and purity. This need for speed, purity, and yield pits these desires against each other as you can typically optimize on only two of the three goals. In this poster, we will describe some techniques that help chemists optimize flash purification and maximize speed, yield, and purity.
Mass-Directed Purification of Natural Products
Natural product chemistry deals with discovering the previously unknown in nature. Compounds found in nature are typically found in low quantity and thus extractions are needed to isolate certain compounds classes or at least compounds with similar solubility.
White Paper A Four Part Approach to Reducing the Environmental Impact of Purification Chromatography
Traditional approaches to compound purification involving chromatography utilize large volumes of relatively toxic and expensive solvents, and significantly contribute to the environmental footprint of organizations involved in molecular research. Current directives for greener chemistry have put pressure on organizations to reduce the environmental impact of their work.
White Paper Reducing the Bottleneck in Target Synthesis
Using the Biotage automated workflow, models have shown that the time taken to produce a target molecule can be reduced by up to eighty percent, allowing project delivery timescales to be reduced accordingly.
Improving Peptide Purification via Flash Column Chromatography by Modulating Mobile Phase pH
Although capable of very high resolution, RP-HPLC is often limited by low column loading capacity, therefore demanding a significant time investment for peptide purification. As an alternative strategy, reversed-phase flash chromatography can also be used to purify synthetic peptides. The larger particle size used in flash column chromatography enables much larger loading capacity, thereby significantly reducing the time required for peptide purification.
Alternative Strategies for Purification of Fully Protected Peptides Using Flash Chromatography
Improvements in solid phase peptide synthesis strategies and development of resin linkages susceptible to low acid cleavage conditions has enabled synthesis of long peptides while keeping the protecting groups intact. This strategy is now used for the preparation of chemically synthesized proteins, wherein shorter peptide fragments are ligated together. They are also found in the synthesis of peptide macrocycles that utilize head-to-tail cyclization strategies. Although linear synthesis of protected peptides is generally straightforward, purification of these compounds using traditional reversed phase methods is quite challenging. Herein we describe the use of normal phase chromatography for purification of fully protected peptides.
INSTRUCTION VIDEOS
Isolera™ Tutorial
WEBINARS
Reversed Phase Purification: Bridging the Gap From Flash to Prep HPLC
News
Media Porosity, Why Should I Care?
"Normal-phase silica has, on average, 50 to 60 Å pores (and a surface area of 500 m²/g). However, for small-molecule reversed-phase chromatography to be useful, the average pore siz...
05-31-2017Scaling Up Flash Purification – Easy as 1, 2, 3, 4
This blog post on the Flash Purification Blog discusses the process of simplified flash purification scale-up. For many chemists performing bench-scale organic synthesis, flash column chro...
11-13-2015New Blog Post: How do I Choose the Right Column Size for Purification by Flash Chromatography?
Techincal specialist Bob Bickler goes through a good method to optimize column size for your purification in his latest blog post on The Flash Purification Blog. "In all my years of work...
09-01-2015Tutorial Video – How to Load Flash Chromatography Cartridges
Biotage® SNAP Ultra flash cartridges allow you to load your samples in seven different ways. No matter what you are purifying - there is always a solution to get the best possible separati...
06-30-2015Why Reusing Flash Cartridges is Bad Science
Regardless of the cartridge brand used, repeated use of silica flash cartridges results in loss of compound resolution and fraction purity. To save money on consumables, many chemists choo...
12-04-2014Isolera™ Used in Recent Natural Products Study
The capabilities of Biotage flash chromatography systems for providing pure compounds from natural products is confirmed in an extensive Korean study of active compounds in the medicinal herb&...
09-12-2014Difficult Fractionation of Lipids Made Easy with Isolera™ Dalton Flash Purification
Fatty acids and their esters are found in many biological substrates. Many of these fats are unhealthy (e.g. saturated fats) while others have been found to be beneficial (e.g. unsaturated, polyuns...
08-06-2014Free Shipping on EU Web Orders
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
07-29-2014The Lines Between Purification and Analysis are Blurring…
By Dr. Greg Saunders, EU Marketing Manager for Organic and Peptide Chemistry at Biotage Liquid chromatography (LC) is used for two different purposes – analytical work and purification &ndas...
03-14-2014Purifying the First Hundred Grams
Preparative FLASH chromatography expedites the production of clinical trial compounds. Tobi Williams is an application chemist specializing in pilot and production purification at Biotag...
10-18-2011Biotage® SNAP Bio
Biotage® SNAP Bio flash cartridges were developed with a small particle size (20 μm) and large pore size (300 Å) to provide increased resolution and the effective separation of complex peptide mixtures.
Biotage® SNAP Ultra
Biotage SNAP Ultra flash chromatography cartridges deliver the highest purification performance available. Packed with Biotage® HP-Sphere™ spherical silica, these cartridges have the highest loading capacity, lowest backpressure and can withstand high flow rates.
Biotage® SNAP Ultra C18
The latest, highest performing reversed phase C18 spherical silica flash cartridges available.
Biotage® SNAP KP-Sil
Biotage SNAP flash purification cartridges are the original recognized industry standard for flash chromatography.
Biotage® SNAP KP-C18-HS
Reversed-phase flash chromatography is a very effective purification technique. Its main application areas include polar, ionizable and highly lipophilic compounds which cannot easily be separated by normal-phase techniques.
Biotage® SNAP KP-NH
Biotage® KP-NH chemistry shields synthetic organic amines from acidic silanols providing improved selectivity, peak shape, purity and yield. Unlike traditional silica and 1° amine (propyl amine) bonded silica, Biotage KP-NH doesn’t require the use of chlorinated solvents or amine additives.
Biotage ZIP® Sphere
Biotage ZIP® Sphere delivers better purification and improved throughput than comparable flash cartridges. The silica surface area has been increased by 40%, meaning higher loading capacity, improved separations, and reduced purification time and solvent use.
Biotage ZIP®
Biotage ZIP® flash cartridges are an evolution in Biotage’s long history of manufacturing flash purification solutions. Biotage ZIP cartridges deliver industry-leading performance that meet the difficult challenges of day to day purification.
Flash 75/150/400 Cartridges
The original flash cartridges invented by Biotage deliver world class purification and ease of use. These industrial scale cartridges provide a straightforward purification scale-up path from other flash cartridges.
