ACI™ and Isolera™ Flash Systems

Time-Efficient Chemistry: Rapid Routes from Synthesis to Isolated Compound (II)

In order to obtain a fast synthesis of an N-unprotected tripeptide, microwave (mw) assisted deprotection, coupling and partial deprotection were applied starting with a purified protected dipeptide [Fig. 1]. The three consecutive mw assisted steps were performed without any intermediate purifications and were accomplished within some 30 minutes. The resulting crude mixture containing the Boc deprotected tripeptide ester was shown to be quite complex [Fig. 2]. The crude product was subjected to automated reversed phase flash chromatography, followed by automated fraction pooling and “thin film evaporation”. The total time for the entire procedure was 1 h 20 min. The purity of the product was > 99%.

Method Development in Flash Purification

Flash purification involves a simple liquid chromatography technique » Method development uses TLC as a way of deciding the parameters for the separation » Isocratic separations are easiest to develop, but gradient separations are more powerful » Software in the Isolera helps with conversion of an isocratic separation to a gradient » It is possible with the Spektra software to run step gradients » Loading options are dependent on the column type » SNAP offers the most flexibility » Care must be taken to choose the best loading option to get good purifications

Quick Guide to Isolera™

Preparation, How Much are You Purifying?

Flash Chromatography: Using UV Detection with UV Absorbing Mobile Phases

The UV absorption spectrum of some solvents overlaps with the product they dissolve, meaning that fraction collection processes cannot distinguish between solvent and product. Luckily, there is technology that solves this problem.

Isolera Software Upgrade Instructions

How to Install Isolera SW 2.3

New Flash System Capabilities Improve Purification Times and Performance

New, higher default flow rates enabled by Isolera ACI systems decreases purification times compared to other systems’ and cartridges’ default flow rate settings without sacrificing separation efficiency. Incorporation of gradient optimization by converting a linear gradient to a step gradient further reduces purification time and solvent use while maintaining separation integrity for a target molecule.

Isolera Assist Quick Guide

Organic Chemistry - Products from Start to Finish

Biotage has developed tools for every step of the organic process, with the entire workflow in mind. This dedicated suite of products vastly expands the range of options in order to truly accelerate discoveries of new molecules for future innovations.

Isolera™ Quick Guide

White Paper A Four Part Approach to Reducing the Environmental Impact of Purification Chromatography

Traditional approaches to compound purification involving chromatography utilize large volumes of relatively toxic and expensive solvents, and significantly contribute to the environmental footprint of organizations involved in molecular research. Current directives for greener chemistry have put pressure on organizations to reduce the environmental impact of their work.

White Paper Reducing the Bottleneck in Target Synthesis

Using the Biotage automated workflow, models have shown that the time taken to produce a target molecule can be reduced by up to eighty percent, allowing project delivery timescales to be reduced accordingly.

Isolera™ Release Notes

White Paper Ein vierstufiges Konzept zur Reduzierung von Umweltbelastungen durch chromatographische Aufreinigung

Bei herkömmlichen Aufreinigungsverfahren mittels Chromatographie kommen große Mengen verhältnismäßig giftiger und teurer Lösungsmittel zum Einsatz, die sich negativ auf den ökologischen Fußabdruck von Unternehmen in der Molekularforschung auswirken. Neue Richtlinien für einen verantwortungsvolleren Umgang mit Chemikalien zwingen Unternehmen, die Umweltauswirkungen ihrer Arbeit zu reduzieren. Durch Einblicke in die Arbeitsabläufe dieser Unternehmen und neue Entwicklungen im Bereich Flash-Chromatographiesysteme und -säulen lassen sich Verbrauch und Toxizität der für die Aufreinigung eingesetzten Lösungsmittel und somit auch die Umweltbelastungen dieser Verfahren reduzieren.

White Paper - Den Engpass in der Target-Synthese überwinden

Modelle haben gezeigt, dass sich die zur Herstellung eines Zielmoleküls benötigte Zeit mit dem automatisierten Workflow von Biotage um bis zu achtzig Prozent verkürzen lässt, sodass auch die Projektlaufzeiten entsprechend verkürzt werden können.