Biotage Purification Instruments are simply the most technologically advanced and most effective purification systems available. Biotage has developed ACI™ Accelerated Chromatographic Isolation, a revolutionary advancement converting regular flash from simple purification to the fastest, greenest, and most economical way to reliably isolate pure compounds from organic synthesis, natural product extracts and other mixes of organic compounds.
ACI™ Accelerated Chromatographic Isolation is an upgrade which works seemlessly with the Isolera Purification family which promises to take chemists to the end point of their purification even faster and even more efficently. ACI is comes as standard on all Isolera systems. Isolera systems already recommend cartridges based on your sample size and TLC data; they can work out the best solvent gradient based on your compound and provide real time information on eluting compounds using UV, ELSD or Mass detection. They substract baseline solvent signals automatically, so what you see is what you get. ACI radically improves this. Traditional purifications taking 15 minutes can be cut down to 5 minutes, with no compromise. 250mg reactions can now be purified in under 3 minutes.
Isolera Dalton opens up new dimensions in automated flash chromatography systems bringing true mass directed fractionation to the chemist. Just dial in your target mass and walk away, the system will retrieve your product out of the mixture you inject.
Isolera™ Spektra is a software upgrade package available for the industry leading Isolera One, Four and LS flash purification systems, with advanced features as standard that are only found on the world’s most expensive high-end chromatography systems. Its innovative algorithms significantly reduce the amount of solvent required to complete the puriifcation and is also fully compatible with ACI systems.
For truly large scale and industrial purification, Biotage Flash 75/150/400 systems provide expeditious scale-up, unsurpassed safety and regulatory compliance. They are the only choice for the separation of multigram to multi Kg quantities of product and are the first choice of pharmaceutical and chemical contract manufacturing companies around the world for critical purification applications.
In order to obtain a fast synthesis of an N-unprotected tripeptide, microwave (mw) assisted deprotection, coupling and partial deprotection were applied starting with a purified protected dipeptide [Fig. 1]. The three consecutive mw assisted steps were performed without any intermediate purifications and were accomplished within some 30 minutes. The resulting crude mixture containing the Boc deprotected tripeptide ester was shown to be quite complex [Fig. 2]. The crude product was subjected to automated reversed phase flash chromatography, followed by automated fraction pooling and “thin film evaporation”. The total time for the entire procedure was 1 h 20 min. The purity of the product was > 99%.
Flash purification involves a simple liquid chromatography technique » Method development uses TLC as a way of deciding the parameters for the separation » Isocratic separations are easiest to develop, but gradient separations are more powerful » Software in the Isolera helps with conversion of an isocratic separation to a gradient » It is possible with the Spektra software to run step gradients » Loading options are dependent on the column type » SNAP offers the most flexibility » Care must be taken to choose the best loading option to get good purifications
Preparation, How Much are You Purifying?
The UV absorption spectrum of some solvents overlaps with the product they dissolve, meaning that fraction collection processes cannot distinguish between solvent and product. Luckily, there is technology that solves this problem.
How to Install Isolera SW 2.3
New, higher default flow rates enabled by Isolera ACI systems decreases purification times compared to other systems’ and cartridges’ default flow rate settings without sacrificing separation efficiency. Incorporation of gradient optimization by converting a linear gradient to a step gradient further reduces purification time and solvent use while maintaining separation integrity for a target molecule.
Biotage has developed tools for every step of the organic process, with the entire workflow in mind. This dedicated suite of products vastly expands the range of options in order to truly accelerate discoveries of new molecules for future innovations.
DMSO and DMF are suitable injection solvents for reversed-phase flash purification. DMSO shows it can be loaded in larger volumes (up to 0.05 mL/g of C18 media or 3.5% of a column volume) without affecting chromatographic separations or carrying compounds with it.
Reversed-phase chromatography is typically used when you need to separate several milligrams of relatively polar compounds that either are not soluble in normal-phase solvents or are not compatible with bare silica because they react, stick, or both. If you are currently using reversed-phase at preparative scale, such as flash chromatography, you know the mobile phase limitations – water with either methanol, acetonitrile, or THF. As with normal-phase flash chromatography, when it comes time to purify you want your crude sample fully solubilized in the weakest possible solvent at the highest possible concentration. ACS 2016
For those chemists performing organic synthesis, reaction mixture purification by flash column chromatography is an integral and necessary part of the synthesis process. However, flash chromatography consumes large volumes of solvent which either needs to be recycled or disposed. ACS 2016.
As reversed-phase flash chromatography gains traction in medicinal chemistry labs the need to monitor its cost and safety are becoming more important. Commonly used reversed-phase solvents typically include water with an organic solvent such as methanol or acetonitrile – each have advantages and disadvantages.
When it comes to polar organic compound purification, many chemists turn to normal-phase flash chromatography often utilizing dichloromethane and methanol as the eluting solvents. While this can work, it often can be challenging to optimize due to methanol’s high polarity and protic chemistry.
For most organic and natural product chemists flash chromatography is a necessary part of their research. As such, many chemists need quick isolation of at least one desired component from a crude mixture in relatively high yield and purity. This need for speed, purity, and yield pits these desires against each other as you can typically optimize on only two of the three goals. In this poster, we will describe some techniques that help chemists optimize flash purification and maximize speed, yield, and purity.
Natural product chemistry deals with discovering the previously unknown in nature. Compounds found in nature are typically found in low quantity and thus extractions are needed to isolate certain compounds classes or at least compounds with similar solubility.
Traditional approaches to compound purification involving chromatography utilize large volumes of relatively toxic and expensive solvents, and significantly contribute to the environmental footprint of organizations involved in molecular research. Current directives for greener chemistry have put pressure on organizations to reduce the environmental impact of their work.
Using the Biotage automated workflow, models have shown that the time taken to produce a target molecule can be reduced by up to eighty percent, allowing project delivery timescales to be reduced accordingly.
Although capable of very high resolution, RP-HPLC is often limited by low column loading capacity, therefore demanding a significant time investment for peptide purification. As an alternative strategy, reversed-phase flash chromatography can also be used to purify synthetic peptides. The larger particle size used in flash column chromatography enables much larger loading capacity, thereby significantly reducing the time required for peptide purification.
Improvements in solid phase peptide synthesis strategies and development of resin linkages susceptible to low acid cleavage conditions has enabled synthesis of long peptides while keeping the protecting groups intact. This strategy is now used for the preparation of chemically synthesized proteins, wherein shorter peptide fragments are ligated together. They are also found in the synthesis of peptide macrocycles that utilize head-to-tail cyclization strategies. Although linear synthesis of protected peptides is generally straightforward, purification of these compounds using traditional reversed phase methods is quite challenging. Herein we describe the use of normal phase chromatography for purification of fully protected peptides.
The capabilities of Biotage flash chromatography systems for providing pure compounds from natural products is confirmed in an extensive Korean study of active compounds in the medicinal herb&...
09-12-2014ACI™ - Accelerated Chromatographic Isolation radically improves the efficiency of the laboratory, embracing the latest developments in purification technology to guide chemists towards improv...
09-09-2014 Flash Purification Has Come of Age Flash purification is a chromatographic technique developed with one goal in mind – to deliver the maximum amount of purified product in the minimum ...
08-07-2014Uppsala, Sweden, 27th June, 2014 – Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, announces the launch of ACI&trad...
06-27-2014By Dr. Greg Saunders, EU Marketing Manager for Organic and Peptide Chemistry at Biotage Liquid chromatography (LC) is used for two different purposes – analytical work and purification &ndas...
03-14-2014Uppsala, Sweden, 1st February, 2013 Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, has announced the launch of an updat...
02-11-2013Uppsala, Sweden, 4th September, 2012 Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, announces that Biotage is in late s...
09-04-2012Dr. Sunil Rana, product manager at Biotage, explains the advanced features for flash chromatography in the new Isolera™ Spektra release. Watch the free webinar recording here >
06-15-201223 April 2012 Product manager Bob Bickler introduces the new time-saving Isolera™ Spektra features for Isolera One, Four and LS Flash chromatography instruments.
04-26-2012Preparative FLASH chromatography expedites the production of clinical trial compounds. Tobi Williams is an application chemist specializing in pilot and production purification at Biotag...
10-18-2011Charlotte, North Carolina, USA, August 16, 2010 – Biotage (STO: BIOT), a leading developer of tools and technology for medicinal and analytical chemistry, announces the introduction of the Is...
08-16-2010Uppsala, Sweden, April 20, 2010 – Biotage (STO: BIOT), a global leader in automated flash purification, tools and technology for medicinal and analytical chemistry, announced it has recently ...
04-21-2010Uppsala, Sweden, February 23, 2010 – Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, announced the launch of enhanced gradient optimization (GO) te...
02-23-2010October 28, 2008 Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, announces the release of its new 2008–09 Synthesis and Purification Catalog. The ...
10-27-2008Isolera™ Spektra is a high performance ACI™ Accelerated Chromatographic Isolation system. Available as Isolera Spektra One, Four and LS, with time, efficiency and cost saving purification run features that are normally only found on the world’s most expensive high-end chromatography systems. With Isolera™ Assist you get the benefits of the feature-rich Isolera Flash Purification Systems in a simple and easy to use interface. It ensures you achieve the best results, regardless of your experience level.
"Simultaneous detection of both positive and negative ions is fantastic. Rarely do samples contain just positively or negatively charged species."
Isolera™ Dalton is the most advanced and innovative flash purification system available, designed by people who understand flash chromatography for the chemists who need it. "Pure convenience! Just pure convenience! It lets us perform purification and analysis at the same time. It gives us tremendous power to isolate unknown compounds.” - Hiroki Nishii, Chugai Pharmaceutical’s Kamakura Research Laboratories
Isolera™ Prime is a value based system which recommends appropriate cartridges and calculates the best solvent conditions and gradients for your purifications. It has solvent pumps, automated fraction collection as standard and delivers much more than just the basics. Solvent use is minimized and advanced compound detection makes sure that your purified compound is delivered at the end of the run. We belive in providing value, so Isolera Prime systems embrace ACI™, a revolutionary advancement, converting regular flash from simple purification to the fastest, greenest, and most economical way to reliably isolate pure compounds for organic synthesis. ACI offerred as standard on Isolera Prime.