This application note describes the extraction of methylmalonic acid from serum, prior to LC-MS/MS analysis. An effective and efficient ISOLUTE® SLE+ protocol optimized for the 200 μL capacity 96-well plate format is used. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs below 10%. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags: 96-well plate, Acidic, Application Notes, Biomarker, Clinical, English, Extrahera, LC-MS/MS, MMA (Methylmalonic acid), Methylmalonic acid (MMA), Serum

This application note describes a simple, effective ISOLUTE® PLD+ protocol for the extraction of methylmalonic acid (MMA) from serum, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags: 96-well plate, Acidic, Application Notes, Biomarker, Clinical, English, Extrahera, LC-MS/MS, MMA (Methylmalonic acid), Methylmalonic acid (MMA), Serum

This application note describes a simple, effective protocol for the extraction of methylmalonic acid (MMA) from serum using ISOLUTE® SAX strong anion exchange solid phase extraction plates, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts. Very clean extracts are possible due to the purely anion exchange retention mechanism available using this silica based sorbent. This minimizes the co-extraction of endogenous interferences through hydrophobic (or non-polar) retention. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags: 96-well plate, Acidic, Application Notes, Biomarker, Clinical, English, Extrahera, LC-MS/MS, MMA (Methylmalonic acid), Methylmalonic acid (MMA), Serum

This application note describes a simple, effective protocol for the extraction of methylmalonic acid (MMA) from serum using EVOLUTE® EXPRESS AX solid phase extraction plates. Samples are prepared using the EVOLUTE EXPRESS load-wash-elute procedure. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags: 96-well plate, Acidic, Application Notes, Biomarker, Clinical, English, Extrahera, LC-MS/MS, MMA (Methylmalonic acid), Methylmalonic acid (MMA), Serum

This application note describes the operating conditions for the automated fractionation of Extractable Petroleum hydrocarbons (EPH) into aliphatic and aromatic (PAH) fractions using the ISOLUTE EPH SPE column in conjunction with the Extrahera automation system.
Tags: Acenaphthene, Acenapthalene, Aliphatic hydrocarbons, Anthracene, Application Notes, Aromatic hydrocarbons, Benzo(a)anthracene, Benzo(a)pyrene, Benzo(b)fluoranthene, Benzo(ghi)perylene, Benzo(k)Fluoranthene, Chrysene, Column, Dibenzo(ah)anthracene, English, Environmental, Extrahera, Fluoranthene, Fluorene, GC-MS, Indeno(123-cd)pyrene, Napthalene, Phenanthrene, Polyaromatic Hydrocarbons (PAH), Pyrene, Soil

Phosphatidylethanol is an alcohol biomarker with a high degree of specificity; blood concentration of PEth correlates to the amount of alcohol consumed. This application note describes the extraction of 3 common species of PEth from whole blood using ISOLUTE® SLE+ supported liquid extraction prior to HPLC-MS/MS analysis. The method is easily automated using the Biotage® Extrahera, and details of the automated procedure are included in the application note.
Tags: 96-well plate, Alcohol metabolite, Application Notes, Clinical, English, Extrahera, Forensic, LC-MS/MS, PEth, Whole blood

This application note describes a simple ISOLUTE® PPT+ protocol for the extraction of methylmalonic acid (MMA) from serum, demonstrating high, reproducible analyte recoveries with low protein content in the extracts. ISOLUTE PPT+ Protein Precipitation plates offer a fast and extremely simple sample preparation approach. Serum proteins are efficiently removed from the sample using an in-plate, ‘solvent first’, protein crash and filter procedure, which is easily automated using the Biotage Extrahera system. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags: 96-well plate, Acidic, Application Notes, Biomarker, Clinical, English, Extrahera, MMA (Methylmalonic acid), Methylmalonic acid (MMA), Serum

This application note describes a protocol for the extraction of 1,25 di-OH Vitamin D2 and 1,25 di-OH Vitamin D3 metabolites from serum using supported liquid extraction prior to LC-MS/MS detection. A calibration range between 5 and 500 pg/mL is demonstrated using a starting volume of 0.25 mL of serum. Sensitivity is maximized through the use of a simple PTAD derivatization and formation of a methylamine complex. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags: 1, 25 Dihydroxyvitamin D, 96-well plate, Application Notes, Clinical, English, Extrahera, LC-MS/MS, Serum, Vitamin D

ISOLUTE® PLD+ Protein and Phospholipid Removal plates offer a substantial improvement in extract cleanliness compared to traditional protein precipitation techniques for bioanalytical sample preparation. This application note describes a simple, effective ISOLUTE® PLD+ protocol for the extraction of 25-hydroxy vitamin D from serum, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts. Included in this application note are the detailed settings for implementing the method on the Biotage Extrahera automation system.
Tags: 25-OH Vitamin D2, 25-OH Vitamin D3, 25-hydroxy-vitamin D, 96-well plate, Application Notes, Clinical, English, Extrahera, LC-MS/MS, Serum, Vitamin D, Vitamins

Biotage® Extrahera™ is a powerful, user friendly automation system for Supported Liquid Extraction (SLE) and Solid Phase Extraction (SPE) based methods, in plate or column formats.
Tags: Brochures

In this catalog product information is displayed with easy-to-follow application details and supporting documentation. Ordering information is found following each product listing.
Tags: Catalogs

In this customer case, Jonathan Tang at University of East Anglia in the UK explains how Biotage sample preparation products have been used successfully in his studies on the human skeleton.
Tags: Customer Cases

Translated versions of the Safety chapter available in the Biotage® Extrahera™ Installation and Safety document (P/N 414157). Languages: Chinese, Danish, Dutch, Finnish, French, German, Greek, Italian, Japanese, Portuguese, Spanish, and Swedish.
Tags: Chinese, Danish, Dutch, Finnish, French, German, Greek, Italian, Japanese, Manuals & User Guides, Portuguese, Spanish, Swedish

The document is for systems with the Extrahera 1.5.4 software installed. Contents: System Overview; Power Failure; Manage Solvents, Sample Types, Plates, Racks, and Pipette Tips; Set Up a Method; Run Single or Multiple Methods; Maintenance; Manual Control; Troubleshooting; Switch Between 24, 48, and 96 Format; General Information
Tags: English, Manuals & User Guides

Tags: English, Manuals & User Guides

This document will assist customers in the preparation of their laboratory for a successful installation of Biotage® Extrahera™.
Tags: Manuals & User Guides

This poster discusses the potential for a single extraction protocol for various drugs of abuse classes in whole blood prior to UPLC-MS/MS analysis. SOFT 2017, Boca Raton also presented at SFTA, Marseille, France, 2018
Tags: Amphetamines, Benzodiazepines, Cannabinoid, Cathinones, Clinical, Cocaines, Column, Drugs of Abuse, English, Extrahera, Forensic, LC-MS/MS, Opiates, Posters, THC metabolites, Whole blood, z-drugs

This poster demonstrates that a large urine panel, comprised of 43 DOAs, from multiple drug classes, can be simultaneously screened by mixed-mode cation exchange SPE (using EVOLUTE EXPRESS CX 96 well plates) despite their disparate intermolecular traits, by thoughtfully selecting appropriate organic wash and elution conditions that simultaneously enable sample isolation and detection along with minimizing sample matrix effects. The extraction method is automated using the Biotage® Extrahera™ Automated sample Preparation Platform. MSACL 2017, Palm Springs SOFT 2017, Boca Raton
Tags: 96-well plate, Benzodiazepines, Carbamates, Carisoprodol, Clinical, Drugs of Abuse, English, Extrahera, Forensic, Gabapentin, Hydrolysed urine, LC-MS/MS, Meprobamate, Opiates, Pain Management, Posters, Pregabalin, Stimulants, Urine

Most drugs are excreted in urine as glucuronide conjugates. Hydrolysis using a beta-glucuronidase enzyme to convert the metabolites to their “free” form for analysis increases sensitivity. Red abalone (Kura Biotech), abalone (Campbell Scientific), and recombinant (IMCSzyme) beta-glucuronidase enzymes were evaluated to determine which provided the most complete hydrolysis of glucuronide metabolites without effecting the overall recovery of non-conjugated compounds. EVOLUTE EXPRESS CX 96-well plates were used to extract hydrolysed urine samples, and the impact of th enzymes was compared. MSACL 2017, Palm Springs SOFT 2017, Boca Raton
Tags: 6-acetylmorphine (MAM), 7-Aminoclonazepam, 96-well plate, Alprazolam, Amitriptyline, Amphetamine, Amphetamines, Analgesics, Barbiturates, Benzodiazepines, Benzoylecgonine, Butalbarbital, Carbamates, Carisoprodol, Chlordiazepoxide, Clinical, Clonazepam, Cocaine, Cocaines, Codeine, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Fentanyl, Forensic, Gabapentin, Hydrocodone, Hydrolysed urine, Hydromorphone, Ketamine, LC-MS/MS, Lorazepam, MDMA, Meperidine, Meprobamate, Methadone, Methamphetamine, Morphine, N-des-tapentadol, Naloxone, Norbuprenorphine, Nordiazepam, Norfentanyl, Norhydrocodone, Norketamine, Normeperidine, Nortryptiline, Opiates, Over the counter pharmaceuticals, Oxazepam, Oxycodone, Oxymorphone, Pain Management, Pharmaceuticals, Phencyclidine (PCP), Phenobarbital, Posters, Pregabalin, Ritalinic acid, Secobarbital, Stimulants, THC metabolites, THC-COOH, Tapentadol, Zolpidem, Zolpidem-phenyl-4-COOH, z-drugs, α-OH alprazolam

This poster discusses the impact of optimization of various parts of the method development process to maximize assay performance while delivering a combined assay for the analysis of urinary catecholamines and metanephrines. The extraction method using EVOLUTE EXPRESS WCX 96-well plates is successfully automated using the Biotage® Extrahera™ Automated Sample Preparation Platform. MSACL 2017, Palm Springs ASMS 2017, Indianapolis
Tags: 3-methoxytyramine, 96-well plate, Catecholamines, Clinical, Dopamine, English, Epinephrine, Extrahera, LC-MS/MS, Metanephrine, Metanephrines, Norepinephrine, Normetanephrine, Posters, Urine

This poster compares the performance of manual processing to a novel automated sample preparation system prior to GC/MS or LC-MS/MS analysis in forensic toxicology applications. Emphasis is placed on the potential for 96-well cross contamination and strategies for its elimination. TIAFT 2016 SOFT 2016
Tags: 2-Hydroxyethylflurazepam, 2-OH-Et-Flurazepam, 6-acetylmorphine (MAM), 7-Aminoclonazepam, 7-Aminoflunitrazepam, 96-well plate, Alprazolam, Amphetamine, Amphetamines, Benzodiazepines, Bromazepam, Buprenorphine, Clinical, Clonazepam, Codeine, Column, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Estazolam, Extrahera, Fentanyl, Flunitrazepam, Flurazepam, Forensic, GC-MS, Hydrocodone, Hydromorphone, Ketamine, LC-MS/MS, Lorazepam, MDA, MDEA, MDMA, Mephedrone, Methadone, Methamphetamine, Midazolam, Morphine, Nitrazepam, Norbuprenorphine, Nordiazepam, Norfentanyl, Norketamine, Opiates, Opiates, Oxazepam, Oxycodone, Oxymorphone, Phencyclidine (PCP), Posters, Temazepam, Triazolam, Urine, Whole blood, Zaleplone, Zolpidem, Zopiclone, z-drugs, α-OH Triazolam, α-OH alprazolam

This poster demonstrates a fast, reliable protocol to extract multiple drug of abuse panels from whole blood using a common supported liquid extraction methodology. This benefits laboratory workflow where multiple assays are run each day, saving both worker hours and consumable costs. SOFT 2015 ASMS 2016 TIAFT 2016
Tags: 2-Hydroxyethylflurazepam, 2C-B, 5-APB, 6-APB, 6-acetylmorphine (MAM), 7-Aminoclonazepam, 7-Aminoflunitrazepam, Alprazolam, Amobarbital, Amphetamine, Anhydroecgonine Methyl Ester AEME, BZP, Benzoylecgonine, Bromazepam, Butabarbital, Butalbarbital, Butylone, Clinical, Clonazepam, Cocaethylene, Cocaine, Cocaines, Codeine, Column, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, Ecgonine Methyl Ester EME, English, Estazolam, Ethylone, Flunitrazepam, Flurazepam, Forensic, GC-MS, Hexobarbital, Hydrocodone, Hydromorphone, Lorazepam, MDA, MDEA, MDMA, MDPV, Mephedrone, Methamphetamine, Methcathinone, Methedrone, Methylone, Midazolam, Morphine, Naphyrone, Nitrazepam, Nordiazepam, Oxazepam, Oxycodone, Oxymorphone, Pentobarbital, Phenobarbital, Posters, Secobarbital, TFMPP, Temazepam, Triazolam, Whole blood, mCPP, pMMA, α-OH Triazolam, α-OH alprazolam

Automated sample preparation using the Biotage®Extrahera™ was compared to an equivalent manual method utilizing a vacuum manifold. Analytes were extracted from pooled stripped plasma using a supported liquid extraction procedure.
Tags: 25-OH Vitamin D2, 25-OH Vitamin D3, 25-hydroxy-vitamin D, 96-well plate, Clinical, English, Extrahera, LC-MS/MS, Plasma, Product Notes, Vitamin D

This product sheet compares automated sample preparation using the Biotage®Extrahera™ to an equivalent manual method utilizing a vacuum manifold. A selection of beta blocker drugs were extracted from pooled stripped plasma using a supported liquid extraction procedure.
Tags: 96-well plate, Alprenolol, Beta blockers, Clinical, English, Extrahera, Forensic, LC-MS/MS, Metoprolol, Pharma, Pindolol, Plasma, Product Notes, Propranolol, β-blockers

This document compares automated sample preparation using the Biotage® Extrahera™ to an equivalent manual method utilizing a vacuum manifold. A selection of non-steroidal anti-inflammatory drugs (NSAIDS) were extracted from pooled stripped plasma using a supported liquid extraction procedure.
Tags: 96-well plate, Clinical, English, Extrahera, Forensic, Indomethacin, Ketoprofen, LC-MS/MS, NSAIDs, Pharma, Plasma, Product Notes, Sulindac

This document details an example of the pipetting precision and accuracy data for four specified volumes across 24 (4 x 6) positions.
Tags: Clinical, Column, English, Extrahera, Forensic, Gravimetric, Pharma, Product Notes

This document details an example of the pipetting precision and accuracy data for four specified volumes across a full 96 well plate.
Tags: 96-well plate, Clinical, English, Extrahera, Forensic, Gravimetric, Pharma, Product Notes

What’s New in Biotage® Extrahera™ 1.5.4
Tags: Release Notes