Evaluation of three beta-glucuronidase enzymes to determine the best hydrolysis conditions for urine samples in clinical toxicology and pain management
Most drugs are excreted in urine as glucuronide conjugates. Hydrolysis using a beta-glucuronidase enzyme to convert the metabolites to their “free” form for analysis increases sensitivity. Red abalone (Kura Biotech), abalone (Campbell Scientific), and recombinant (IMCSzyme) beta-glucuronidase enzymes were evaluated to determine which provided the most complete hydrolysis of glucuronide metabolites without effecting the overall recovery of non-conjugated compounds.
EVOLUTE EXPRESS CX 96-well plates were used to extract hydrolysed urine samples, and the impact of th enzymes was compared.
MSACL 2017, Palm Springs
SOFT 2017, Boca Raton
Tags:
6-acetylmorphine (MAM),
7-Aminoclonazepam,
96-well plate,
Alprazolam,
Amitriptyline,
Amphetamine,
Amphetamines,
Analgesics,
Barbiturates,
Benzodiazepines,
Benzoylecgonine,
Butalbarbital,
Carbamates,
Carisoprodol,
Chlordiazepoxide,
Clinical,
Clonazepam,
Cocaine,
Cocaines,
Codeine,
Diazepam,
Dihydrocodeine (DHC),
Drugs of Abuse,
EDDP,
English,
Fentanyl,
Forensic,
Gabapentin,
Hydrocodone,
Hydrolysed urine,
Hydromorphone,
Ketamine,
LC-MS/MS,
Lorazepam,
MDMA,
Meperidine,
Meprobamate,
Methadone,
Methamphetamine,
Morphine,
N-des-tapentadol,
Naloxone,
Norbuprenorphine,
Nordiazepam,
Norfentanyl,
Norhydrocodone,
Norketamine,
Normeperidine,
Nortryptiline,
Opiates,
Over the counter pharmaceuticals,
Oxazepam,
Oxycodone,
Oxymorphone,
Pain Management,
Pharmaceuticals,
Phencyclidine (PCP),
Phenobarbital,
Posters,
Pregabalin,
Ritalinic acid,
Secobarbital,
Stimulants,
THC metabolites,
THC-COOH,
Tapentadol,
Zolpidem,
Zolpidem-phenyl-4-COOH,
z-drugs,
α-OH alprazolam
Part No: P156
Published: 2017
0.23 MB
Format: pdf
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