The objective of this study was to determine feasibility of transferring a validated liquid-liquid extraction method to a supported liquid extraction method using Biotage ISOLUTE® SLE+ 96-well plates (400μL sample capacity). The laboratory supporting this study processes ~550 samples per month supporting vitamin D analysis in plasma and serum (mixed samples/not separated). During this evaluation, it was determined that sample processing time was reduced by ~50% for a de-identified patient set (n=30, split and processed by both methods). Towards high throughput analysis platforms, ISOLUTE SLE+ was demonstrated as a viable solution in the sample preparation workflow for vitamin D applications.
This study was performed In conjunction with collaborators at the Seattle Children’s Hospital.
Biotage Industrial Flash Cartridges Explained
Finally - an Easy Urine Sample Preparation for LC/MS Analysis
A Smorgasbord of Analytical Methods
Extraction of Acidic Catecholamine Metabolites in Plasma Using EVOLUTE® EXPRESS ABN Prior to LC-MS/MS Analysis
Seminar: An Introduction to the Biotage Automated Workflow Solution