The objective of this study was to determine feasibility of transferring a validated liquid-liquid extraction method to a supported liquid extraction method using Biotage ISOLUTE® SLE+ 96-well plates (400μL sample capacity). The laboratory supporting this study processes ~550 samples per month supporting vitamin D analysis in plasma and serum (mixed samples/not separated). During this evaluation, it was determined that sample processing time was reduced by ~50% for a de-identified patient set (n=30, split and processed by both methods). Towards high throughput analysis platforms, ISOLUTE SLE+ was demonstrated as a viable solution in the sample preparation workflow for vitamin D applications.
This study was performed In conjunction with collaborators at the Seattle Children’s Hospital.
Biotage Goes Direct in Italy
New Peptide Synthesis Workflow Brochure
Extraction of Plasma Catecholamines and Metanephrines Using EVOLUTE® EXPRESS WCX SPE Prior to LC-MS/MS Analysis
New Second Generation Solvent Evaporators from Biotage
Extraction of Tobacco-Specific Nitrosamines (TSNAs) from Urine Using ISOLUTE® SLE+ Prior to UPLC/MS/MS Analysis