This method achieves highly reproducible recoveries for 1,25 diOH Vitamins D2 and D3. A calibration range between 5 and 500 pg/mL is demonstrated using a starting volume of 0.25 mL of serum. Sensitivity is maximized through the use of a simple PTAD derivatization and formation of a methylamine complex.
The method was automated on the Biotage® Extrahera™, using ISOLUTE SLE+ 400 μL Supported Liquid Extraction plates. Full details of system setting are included in the application note.
Download the application note below.
How to Develop a Scalable Metals Removal Process in Chemistry
Extraction of Phosphatidylethanol Species from Whole Blood Using ISOLUTE® SLE+
Automated Fractionation of Extractable Petroleum Hydrocarbons (EPH) in Soil
Current Methodologies for Drugs of Abuse Urine Testing
The Smart Solution to Regulatory Compliance for Metals in Pharma