This method achieves highly reproducible recoveries for 1,25 diOH Vitamins D2 and D3. A calibration range between 5 and 500 pg/mL is demonstrated using a starting volume of 0.25 mL of serum. Sensitivity is maximized through the use of a simple PTAD derivatization and formation of a methylamine complex.
The method was automated on the Biotage® Extrahera™, using ISOLUTE SLE+ 400 μL Supported Liquid Extraction plates. Full details of system setting are included in the application note.
Download the application note below.
Extraction of Thyroid Hormones: T3, rT3 and T4 from Serum Using EVOLUTE® EXPRESS AX Plates Prior to LC-MS/MS Analysis
Extraction of Vitamin B7 (Biotin) from Serum Using EVOLUTE® EXPRESS ABN
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