This method achieves highly reproducible recoveries for 1,25 diOH Vitamins D2 and D3. A calibration range between 5 and 500 pg/mL is demonstrated using a starting volume of 0.25 mL of serum. Sensitivity is maximized through the use of a simple PTAD derivatization and formation of a methylamine complex.
The method was automated on the Biotage® Extrahera™, using ISOLUTE SLE+ 400 μL Supported Liquid Extraction plates. Full details of system setting are included in the application note.
Download the application note below.
Finally - an Easy Urine Sample Preparation for LC/MS Analysis
A Smorgasbord of Analytical Methods
Extraction of Acidic Catecholamine Metabolites in Plasma Using EVOLUTE® EXPRESS ABN Prior to LC-MS/MS Analysis
Seminar: An Introduction to the Biotage Automated Workflow Solution
The 2018 Analytical Catalog is Here