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Two New Application Notes for Extraction of 1-nor-9-Carboxy-Δ9-THC from Hydrolyzed Urine

28 August 2018

These two new application notes from Biotage describe the fully automated extraction of carboxy-THC from urine, following base hydrolysis prior to GC/MS analysis. The methods were automated using Biotage® Extrahera™ configured for use with either ISOLUTE® SLE+,(Application Note No. AN892) or EVOLUTE® EXPRESS ABN (Application Note AN893).

 

Application Note Number AN892 Using ISOLUTE® SLE+.

This simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 10% for carboxy-THC and its deuterated internal standard. Using Biotage® Extrahera™, 24 samples are extracted in approximately 35 minutes. Limit of quantitation is below the SAMHSA/EWDTS confirmation cut off of 15 ng/mL for workplace testing applications.

Download Application Note AN892

 

Application Note Number AN893 Using EVOLUTE® EXPRESS ABN.

This method delivers clean extracts and analyte recovery of 90% with RSD lower than 10% for carboxy-THC and its deuterated internal standard. Using the Biotage® Extrahera™, 24 samples were extracted in approximately 38 minutes. The limit of quantitation of 2 ng/mL is below the SAMHSA/ EWDTS confirmation cut off of 15 ng/mL for workplace testing applications.

Download Application Note AN893

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