The protocol involves sample collection as per Oral-Eze intructions, vortex mixing with ammonium hydroxide and simple extraction with ISOLUTE® SLE+ 400 μL plates or 1 mL columns. RSD’s ranged from 1.2%–9.1%.
ISOLUTE® SLE+ Supported Liquid Extraction columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.
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Application Video: Extraction Steroids from Serum with ISOLUTE® SLE+