A poster (P165) presented at ASMS 2017, Indianapolis, discusses three sample preparation methodologies for 52 drug analytes, and presents evaluations of recovery, matrix effects and process efficiency.
While “dilute and shoot” methods are easy and affordable, they can result in shortened LC column lifetimes and increased MS instrumentation downtime. Matrix effects can also effect sensitivity and overall method performance. Supported Liquid extraction (SLE) and Solid Phase Extraction (SPE) provide cleaner samples for LC-MS/MS and more robust clinical assays.
Successful sample preparation method development requires understanding the retention mechanism of the analytes in the assay. The octanol-water partition coefficient (logP) and acid dissociation constant (pKa) of the compounds of interest are important properties that can guide sample preparation strategy and optimization of method conditions.
Convert Your TurboVap® LV Into a TurboVap® II
New Findings presented at ASMS 2018
Webinar: A Roadmap to Successful Flash Chromatography
Evaporate Water from Reaction Mixtures in Less Than 10 Minutes
Evaporation of Heat-sensitive Compounds