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Peptide Purification Using High Performance Flash Chromatography

02 November 2017

The purification step is one of the main bottlenecks in the peptide synthesis workflow. Preparative reversed-phase HPLC is normally the method of choice for purification of synthetic peptides, but is limited by small loading amounts, long separation times, poor recoveries and high costs. Could anything else be used to achieve the necessary purity while maintaining a desirable yield and throughput?

 

A poster from Biotage in collaboratin with the University of Copenhagen, presented at two peptide conferences during October in Australia, shows that high performance flash chromatography (HPFC) can be used as an efficient technique for the purification of synthetic peptides. The poster presented data showing examples where high performance flash chromatography is used either as the sole method of peptide purification or as part of a multi-step process to initially remove most of the undesired impurities by HPFC prior to a final “polishing” step by RP-HPLC.

High performance flash chromatography allows more peptide to be processed in a single injection and significant amount of impurities can be removed that would require more time and effort to purify by RP-HPLC alone.

The poster was presented at:

  • 6th Modern Solid Phase Peptide Synthesis & Its Applications Symposium, Kingfisher Bay Resort, Fraser Island, Queensland, Australia
  • 12th Australian Peptide Conference, Peppers Noosa Resort, Queensland, Australia

Download the full poster below