A poster from Biotage in collaboratin with the University of Copenhagen, presented at two peptide conferences during October in Australia, shows that high performance flash chromatography (HPFC) can be used as an efficient technique for the purification of synthetic peptides. The poster presented data showing examples where high performance flash chromatography is used either as the sole method of peptide purification or as part of a multi-step process to initially remove most of the undesired impurities by HPFC prior to a final “polishing” step by RP-HPLC.
High performance flash chromatography allows more peptide to be processed in a single injection and significant amount of impurities can be removed that would require more time and effort to purify by RP-HPLC alone.
The poster was presented at:
Extraction of Acidic Catecholamine Metabolites in Plasma Using ISOLUTE® SLE+ Prior to LC-MS/MS Analysis
Video Demonstration: Multi Racks for TurboVap®
Effective Purification of Peptides using Normal Phase Flash
Webinar Recording - Advances in Sample Preparation Using In-Well Hydrolysis for Urine Samples
Video Demonstration - the Software in Turbovap® Evaporators