Four new posters by Biotage R&D and Application Scientists have been presented this week at HPLC 2014, New Orleans, LA, USA.
The studies focus on clean-up of biofluids using different sample preparation techniques, and how sample preparation is an important aspect of any analytical method. A number of different approaches are covered that can be used to remove the major endogenous components - particularly proteins and phospholipids - and may impact the quality of HPLC methods and data.
P77 Sample Preparation and Protein: A Comparison of Protein Removal with Various Sample Preparation Techniques using Gel Electrophoresis
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
P78 Effective Strategies for Phospholipid Removal using Polymer-based Solid Phase Extraction (SPE) and LC-MS/MS Analysis
This poster focuses on the different strategies that can be employed when using EVOLUTE SPE sorbents (with both single and dual retention mechanisms) to reduce or eliminate residual phospholipids in sample extracts.
P79 Evaluation of a Novel 96-well Filter Plate for the Effective Removal of Serum Proteins and Phospholipids prior to LC-MS/MS Analysis
The new ISOLUTE® PLD+ Protein and Phospholipid Removal Plate is highlighted in this poster. Protein and phospholipid removal, and analyte recovery are included, along with data illustrating the positive impact of clean (PL and protein free) samples in maintaining analyte signal intensity and low UPLC column back pressure over multiple LCMS runs.
P80 Supported Liquid Extraction (SLE): A Novel Sample Preparation Method for Small Molecule Extraction from Biological Matrices Prior to High Pressure Liquid Chromatography Mass Spectrometry Analysis
This poster describes the benefits of supported liquid extraction and highlights its use in removal of endogenous matrix components that cause ion suppression/enhancement (matrix effects) in LC-MS/MS analyses.
All posters are available for download at the literature library.
White Paper: Reduce Solvent Use by 50%
Perfecting Your Mass Spectrometer Parameters
Sample Preparation Strategies for Urine Panels with 50 Drugs
Biotage Synthesizer Peer-reviewed Literature
How to Identify Compounds in a Mass Spectrum