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New Application: Extraction of Barbiturates from Oral Fluid

05 June 2014

A new Application Note from Biotage was released today describing the extraction of Butalbarbital, Butabarbital, Amobarbital, Pentobarbital, Secobarbital, Hexobarbital and Phenobarbital from oral fluid matrix collected using the Oral-Eze® collection devices, prior to GC/MS analysis.

ISOLUTE® SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquidliquid extraction (LLE) for bio-analytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.

This application note describes an effective and efficient ISOLUTE SLE+ protocol optimized for the 400 μL sample capacity column format. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 95% with RSDs lower than 9% for all analytes.

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