Structural and functional studies of membrane proteins remain a challenge due to difficulties in their handling. The combination of using high concentrations of reagents (0.5M) and fast and precise microwave heating to increase the coupling efficiency using the Biotage® Initiator+ Alstra™ microwave peptide synthesizer, enabled the efficient synthesis of peptide ‘18A’ and simplified the subsequent purification by RP-HPLC despite its relatively low solubility, to afford the desired peptide in 52% yield (468 mg). Read the full application note using the link below.
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