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Oral drug test with ISOLUTE SLE+

Extraction Protocol for 47 Drugs of Abuse

16 January 2015

A new application note from Biotage describes the extraction of 47 drugs of abuse and metabolites from oral fluid matrix collected using the Intercept Oral Fluid Drug Test Kit (from Orasure Technologies), prior to UPLC-MS/MS analysis. 

 

ISOLUTE® SLE+ Supported Liquid Extraction columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation. This application note describes an efficient ISOLUTE SLE+ protocol for 47 drugs of abuse, with analyte recovery exceeding 90%. 

Analytes

2-OH-ethyl-flurazepam 
6-MAM 
6-MAM-D3 
7-amino-clonazepam 
7-amino-flunitrazepam 
Alprazolam 
Amphetamine 
Amphetamine-D5 
Bromazepam 
Buprenorphine 
BZE 
BZE-D3 
Clonazepam 
Cocaine 
Codeine 
Diazepam 
Diazepam-D5 
Dihydrocodeine

EDDP 
Estazolam 
Fentanyl 
Flunitrazepam 
Flurazepam 
Hydrocodone 
Hydromorphone 
Ketamine 
Lorazepam 
MDA 
MDEA 
MDMA 
Mephedrone 
Methadone 
Methamphetamine
Midazolam 
Morphine  

Morphine-D3 
Nitrazepam 
Norbuprenorphine 
Nordiazepam 
Norfentanyl 
Norketamine 
Oxazepam 
Oxycodone 
Oxymorphone 
PCP 
Temazepam 
Triazolam 
Zaleplone 
Zolpidem 
Zopiclone 
α-OH-alprazolam 
α-OH-triazolam




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