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Extraction of Thyroid Hormones: T3, rT3 and T4 from Serum Using EVOLUTE® EXPRESS AX Plates Prior to LC-MS/MS Analysis

26 April 2017

This application note describes a polymer-based strong anion exchange mixed-mode SPE protocol for the extraction of T3, rT3 and T4 from serum prior to LC-MS/MS analysis.


The method described in this application note demonstrates selective extraction of the thyroid hormones T3, rT3 and T4 from serum. 200 µL of serum was extracted using the EVOLUTE® EXPRESS AX 30 mg fixed well plate format. High reproducible recoveries and low phospholipid content were observed, demonstrating limits of quantitation < 50 pg/mL.

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Analytes

  • Tri-iodothyronine (T3)
  • reverse Tri-iodothyronine (rT3)
  • Thyroxine (T4)

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