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Extraction of Phosphatidylethanol Species from Whole Blood Using ISOLUTE® SLE+

21 March 2017

This simple sample preparation procedure produces clean extracts, good recoveries with low RSD, and LOQ from 20 ng/mL. This method can be automated using Biotage® Extrahera™. The application note contains an appendix with details.

 

Phosphatidylethanol (PEth) is an alcohol biomarker with a high degree of specificity; blood concentration of PEth correlates to the amount of alcohol consumed. This application note describes the extraction of three common species of PEth from whole blood using ISOLUTE® SLE+ supported liquid extraction prior to HPLC-MS/MS analysis. This simple sample preparation procedure produces clean extracts, good recoveries with low RSD, and LOQ from 20 ng/mL, and can be fully automated using Biotage® Extrahera™. 96 samples can be processed in 22 minutes and 33 seconds.

ISOLUTE SLE+ supported liquid extraction fixed-well plates offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation time.

Download Application Note Below »

 

Analytes

  • 1,2-dipalmitoyl-sn-glycero-3-phosphoethanol (PEth-16:0/16:0)
  • 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanol (PEth-16:0/18:1)
  • 1,2-dioleoyl-sn-glycero-3-phosphoethanol (PEth-18:1/18:1)
  • D5 -1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanol (D5-PEth-16:0/18:1) was used as an internal standard.

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