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Extraction of Oxytocin and Vasopressin from Serum Using EVOLUTE® EXPRESS ABN Prior to LC-MS/MS Analysis

19 May 2017

The method described in this application note achieves high reproducible extraction recoveries of the peptides oxytocin and vasopressin from serum using a fast, simple EVOLUTE® EXPRESS Load-Wash-Elute SPE procedure.

 

Extraction from serum was performed using EVOLUTE EXPRESS ABN 96-well plates, utilizing a highly aqueous elution solvent, and minimizing co-extractable material in the form of proteins, lipids and phospholipids.

 

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Analytes

  • Oxytocin and Vasopressin

     

 

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