The protocol described is optimized for the 1 mL capacity ISOLUTE SLE+ column format. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 70% with RSDs lower than 10% for all analytes.
ISOLUTE® SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.
Download the application note below.
This concludes the Biotage Application Note series covering extraction of all the major drug classes from NeoSal oral fluid collection devices.
SPE Protocol for Extraction of Urinary Catecholamines and Metanephrines
Extraction of Benzodiazepines From Oral Fluid Using ISOLUTE® SLE+
A Beginner's Guide to Flash Purification of Peptides
How Solvent Choice Impacts Flash Chromatography Performance
Extraction of Teicoplanin from Plasma Using EVOLUTE® EXPRESS ABN Prior to HPLC-DAD Analysis