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AN885 Amphetamines

Extraction of Amphetamines and Analogues from Hair Using ISOLUTE® SLE+ Prior to GC/MS Analysis

15 June 2017

This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization.


This application note describes an effective and efficient ISOLUTE® SLE+ protocol optimized for 1 mL capacity formats. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 72% with RSDs lower than 10% for all analytes.

In addition, this note describes how to achieve quantitation of all analytes down to 0.2 ng/mg of hair. This complies with the LOQ values for laboratories involved in amphetamine testing, as suggested by the Society of Hair Testing (SoHT).

 

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Analytes

  • Amphetamine
  • Methamphetamine
  • MDA
  • MDMA
  • MDEA

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