This application note details effective protocols for optimized for loading volumes of either 400 μL or 1 mL. The simple sample preparation procedure delivers clean extracts and analyte recoveries ranging from 85–99% with RSDs below 10% for all analytes.
ISOLUTE® SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.
Cocaine, AEME, EME, Benzoylecgonine, Cocaethylene and Norcocaine.
Typical analyte % recoveries for extracted cocaine and metabolites from urine (n=7) using the described ISOLUTE® SLE+ protocol.
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Extraction of Plasma Catecholamines and Metanephrines Using EVOLUTE® EXPRESS WCX SPE Prior to LC-MS/MS Analysis
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Extraction of Tobacco-Specific Nitrosamines (TSNAs) from Urine Using ISOLUTE® SLE+ Prior to UPLC/MS/MS Analysis