Whole blood continues to be a valuable tool in forensic toxicology for the immediate and near-term detection of illicit drugs, and in cases where no other sample is available. Screening drugs of abuse can be complicated due to the wide variation of functional groups associated with different analyte classes. Most extraction techniques cannot extract all analytes using a single procedure without using non-optimal extraction protocols resulting in compromised extract cleanliness. Supported liquid extraction allows for the simultaneous analysis of cross functional analytes in a single extraction protocol without forfeiting extract cleanliness.
ISOLUTE® SLE+ 1 mL capacity ‘C’ columns; PN. 820-0140-C
400 μL of whole blood was pre-treated with 400 μL of 1% ammonium hydroxide in water (NH4OH)
750 μL of pre-treated blood was applied to the columns
2 x 2.5 mL aliquots of DCM. Each aliquot was allowed to flow under gravity for 5 minutes before applying a pulse of positive pressure for 10-20 seconds to completely remove the final aliquot.
The extracts, with the exception of amphetamine compounds, were evaporated to dryness at 40 °C. Amphetamines were evaporated at ambient temperature. Subsequent analysis was conducted with GC/MS.
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