As the popularity of post-synthetic peptide chemistry continues to go, additional purification strategies will be required. Standard reversed phase techniques may be ineffecient due to the extreme hydrophobicity imbued by the presence of sidechain protecting groups. Herein, we demonstrate that normal phase chromatography, specifically the use of a methanol:DCM solvent system and a step gradient, can indeed facilitate rapid purification of fully protected peptides.
Biotage Acquires Horizon Technology
Extraction of Acidic Catecholamine Metabolites in Plasma Using ISOLUTE® SLE+ Prior to LC-MS/MS Analysis
Video Demonstration: Multi Racks for TurboVap®
Effective Purification of Peptides using Normal Phase Flash
Webinar Recording - Advances in Sample Preparation Using In-Well Hydrolysis for Urine Samples