This poster compares the use of supported liquid extraction (ISOLUTE® SLE+) and a novel protein and phospholipid depletion plate (ISOLUTE® PLD+), for the extraction of 25-hydroxy vitamin D. The extraction protocols were ultimately transferred to an SPE automation platform and method performance versus manual processing compared.
Vitamin D deficiency can result in various health issues such as osteoporosis, liver and kidney problems, and is associated with increased risk of cancers and multiple sclerosis. From this standpoint vitamin D analysis has extremely important clinical relevance. Many sample preparation approaches to the extraction of 25-hydroxy vitamin D have been employed prior to LC-MS/MS analysis. Simple protein precipitation, supported liquid extraction and complex SPE methodology are all in routine use.
This poster was first presented at MSACL 2015, Salzburg.
Extraction of Amphetamines and Analogues from Hair Using ISOLUTE® SLE+ Prior to GC/MS Analysis
Four Posters Presented at ASMS, Indianapolis
Video Tip: What is ISOLUTE® Filter+
Media Porosity, Why Should I Care?
Extraction of Polyfluorinated Compounds in Water Using EVOLUTE® EXPRESS WAX SPE Prior to LC-MS/MS Analysis