In developing the EVOLUTE® EXPRESS SPE family, Biotage’s goal has been to improve productivity during method development, validation and routine analysis with a combination of sorbents, components and methods that can be applied to as wide a range of analytes as possible, and at the same time reduce or eliminate unwanted matrix components from the final extracts. Whether you are working in the field of bio-analysis during drug development, clinical or forensic toxicology, or with the diversity of samples in food safety and environmental applications, the tools and methods used for sample preparation have a clear impact on productivity.
The narrow pore size of all EVOLUTE® EXPRESS sorbents eliminate proteins from the final extract by preventing them from retaining during the load step.
The EVOLUTE® EXPRESS product family is versatile, available in five sorbent chemistries. Unlike silica-based sorbents, EVOLUTE EXPRESS products are deconditioning resistant, meaning you will never experience poor analyte-sorbent interactions due to phase collapse. In addition they further benefit from Biotage’s Load-Wash-Elute protocols. Unlike traditional silica-based SPE sorbents, which cannot perform under extremely high or low pH, EVOLUTE EXPRESS products are pH resistant so you can work without limitations.
EVOLUTE EXPRESS mixed-mode sorbents consist of a modified non-polar polystyrenedivinylbenzene polymer backbone which incorporates polar hydroxyl groups. These non-ionizable hydroxyl groups ensure that the polymer is both highly water wettable, and also able to extract a diverse range of analytes through non-polar (van der Waals) interactions. No secondary interactions exist, so EVOLUTE EXPRESS retention and elution characteristics are completely predictable and extractions on these robust sorbents are not adversely affected by drying of the sorbent bed during sample processing.
We’ve eliminated the need to condition and equilibrate with Load-Wash-Elute protocols.
We use frit components that enhance flows even for viscous samples.
We’ve developed proven generic methods and optimization tips that really work.
EVOLUTE EXPRESS sorbents have protein exclusion built in by design.
Our wash and elution protocols reduce or eliminate matrix interferences.
We make sure EVOLUTE EXPRESS products won’t contaminate your samples.
Extraction of Thyroid Hormones: T3, rT3 and T4 from Serum Using EVOLUTE® EXPRESS AX Plates Prior to LC-MS/MS Analysis
Extraction of Vitamin B7 (Biotin) from Serum Using EVOLUTE® EXPRESS ABN
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